Cholinesterase (CHE) Activity Assay Kit
- SKU:
- MAES0210
- Product Type:
- Assay
- Instrument:
- Microplate Reader
- Detection Method:
- Colorimetric
- Sample Type:
- Serum, Plasma, Animal Tissue
- Sensitivity:
- 2.17 U/mL
- Range:
- 2.17-71.33 U/mL
- Assay Time:
- 60 min
Frequently bought together:
Description
Product Name: | Cholinesterase (CHE) Activity Assay Kit |
Product Code: | MAES0210 |
Sample Type: | Serum, Plasma, Animal Tissue |
Assay Time: | 60 min |
Assay Type: | Enzyme Activity |
Synonyms: | CHE |
Detection Instrument: | Microplate reader (505-535 nm, optimum wavelength: 520 nm) |
Detection Method: | Colorimetric method |
Sensitivity: | 2.17 U/mL |
Detection Range: | 2.17-71.33 U/mL |
Inter-assay CV: | 0.064 |
Intra-assay CV: | 0.024 |
Cholinesterase breaks down acetylcholine into choline and acetic acid. Acetylcholine that is not hydrolyzed by cholinesterase reacts with hydroxylamine to form acetamidamine. It reacts in an acidic solution to form a brown-red hydroxamate iron complex. The color depth is directly proportional to the amount of remaining acetylcholine, which can be colorimetrically quantified. Cholinesterase activity was calculated.
Storage: | 2-8°C |
Valid Period: | 12 months |
Other Reagents Required: | Normal saline (0.9% NaCl) |
Other Instruments Required: | EP tube, centrifuge, Vortex mixer, 37℃ water bath, Microplate reader |