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Choline Acetyltransferase Activity Assay Kit (Tissue Samples) (MAES0101)

SKU:
MAES0101
Product Type:
Assay
Instrument:
Microplate Reader
Sample Type:
Tissue
Sensitivity:
1.21 U/g fresh weigh
Range:
1.21-40 U/g fresh we
Assay Time:
65 min
Shipping:
Gel Pack
Research Area:
Diabetes & Obesity
€409
Frequently bought together:

Description

system_update_altDatasheet
Product Name:Choline Acetyltransferase Activity Assay Kit (Tissue Samples)
Product Code:MAES0101
Product Size:96 Assays
Sample Type:Animal tissue
Assay Time:65 min
Instrument:Microplate reader
Sensitivity:1.21 U/g fresh weigh
Detection Range:1.21-40 U/g fresh we
Inter CV:9.4
Inter CV:5.1

Choline acetyltransferase (ChAT, EC 2.3.1.6) is an enzyme for biosynthesis of acetylcholine, which generates acetylcholine by catalyzing acetyl group transfer from acetyl coenzyme A to choline. ChAT is synthesized in the perinuclear body of neurons and transported to neurons through the mechanism of slow axon transport. ChAT is the most specific indicator for monitoring the functional status of cholinergic neurons in central and peripheral nervous systems.

Acetyl-CoA can react with choline under the catalysis of choline acetyltransferase (ChAT) to produce coenzyme A (CoA), CoA can combine with the 4, 4-dithiopyridine. The activity of ChAT can be calculated indirectly by measuring the OD value at 324 nm.

Shelf Life:3 Months
Storage:Reagent 2, 3, 6 -20°C;, Others: 2-8°C
Shipping:Gel Pack
Research Area:Enzymes, Others

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