The CHMP4B Polyclonal Antibody (CAB7402) is a valuable tool for researchers studying CHMP4B, a protein involved in cellular processes such as endosomal sorting and cytokinesis. This antibody, produced in rabbits, exhibits high specificity for CHMP4B and is validated for use in various applications, including Western blotting.CHMP4B, part of the ESCRT-III complex, plays a crucial role in the final stages of cytokinesis and multivesicular body (MVB) formation.
Dysregulation of CHMP4B has been linked to diseases such as cancer and neurodegenerative disorders, making it a promising target for therapeutic interventions. By using the CHMP4B Polyclonal Antibody, researchers can study the expression and localization of CHMP4B in different cell types, providing valuable insights into its function and potential implications for disease pathology.
Product Name:
CHMP4B Rabbit Polyclonal Antibody
SKU:
CAB7402
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-224 of human CHMP4B (NP_789782.1).
Cytoplasm, Late endosome membrane, Peripheral membrane protein, cytosol.
Calculated MW:
25kDa
Observed MW:
25kDa
This gene encodes a member of the chromatin-modifying protein/charged multivesicular body protein (CHMP) protein family. The protein is part of the endosomal sorting complex required for transport (ESCRT) complex III (ESCRT-III), which functions in the sorting of endocytosed cell-surface receptors into multivesicular endosomes. The ESCRT machinery also functions in the final abscisson stage of cytokinesis and in the budding of enveloped viruses such as HIV-1. The three proteins of the CHMP4 subfamily interact with programmed cell death 6 interacting protein (PDCD6IP, also known as ALIX), which also functions in the ESCRT pathway. The CHMP4 proteins assemble into membrane-attached 5-nm filaments that form circular scaffolds and promote or stabilize outward budding. These polymers are proposed to help generate the luminal vesicles of multivesicular bodies. Mutations in this gene result in autosomal dominant posterior polar cataracts.
Purification Method:
Affinity purification
Gene ID:
128866
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from Mouse spleen using CHMP4B Rabbit pAb(CAB7402) at 1:400 dilution.Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection:ECL Basic Kit (AbGn00020).Exposuretime:90s.
