The CHMP2B Polyclonal Antibody (CAB5399) is designed for research involving CHMP2B, a protein involved in the ESCRT-III complex and essential for endosomal sorting and membrane abscission during cell division. The antibody, raised in rabbits, is highly reactive with human samples and is validated for use in immunofluorescence and immunohistochemistry applications. It binds specifically to the CHMP2B protein, enabling visual detection and localization within cells, making it ideal for studies in cell biology and neuroscience research.
CHMP2B is known to be associated with neurodegenerative diseases such as frontotemporal dementia and amyotrophic lateral sclerosis. Its role in cellular processes and disease pathology makes it a valuable target for research into these devastating conditions. Understanding the function of CHMP2B is crucial for unraveling the mechanisms underlying neurodegeneration and developing therapeutic interventions to combat these diseases.
Product Name:
CHMP2B Rabbit Polyclonal Antibody
SKU:
CAB5399
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-213 of human CHMP2B (NP_054762.2).
Cytoplasm, Late endosome membrane, Peripheral membrane protein, cytosol.
Calculated MW:
24kDa
Observed MW:
28kDa
This gene encodes a component of the heteromeric ESCRT-III complex (Endosomal Sorting Complex Required for Transport III) that functions in the recycling or degradation of cell surface receptors. ESCRT-III functions in the concentration and invagination of ubiquitinated endosomal cargos into intralumenal vesicles. The protein encoded by this gene is found as a monomer in the cytosol or as an oligomer in ESCRT-III complexes on endosomal membranes. It is expressed in neurons of all major regions of the brain. Mutations in this gene result in one form of familial frontotemporal lobar degeneration.
Purification Method:
Affinity purification
Gene ID:
25978
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using CHMP2B antibody (CAB5399) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Basic Kit (AbGn00020)._Exposure time: 30s.
