The CHMP2B Polyclonal Antibody (CAB13410) is developed for researchers investigating CHMP2B, a key protein involved in the endosomal sorting complex required for transport (ESCRT) pathway. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blotting applications. By specifically binding to the CHMP2B protein, this antibody allows for precise detection and analysis in a variety of cell types, making it well-suited for studies in cell biology and neurodegenerative diseases.
CHMP2B plays a crucial role in the ESCRT machinery, which is responsible for sorting and trafficking of proteins within cells. Dysregulation of this pathway has been implicated in neurodegenerative disorders such as frontotemporal dementia and amyotrophic lateral sclerosis. The CHMP2B Polyclonal Antibody offers researchers a valuable tool for investigating the function and dysfunction of CHMP2B in these conditions, ultimately advancing our understanding of their underlying mechanisms and potential therapeutic targets.
Product Name:
CHMP2B Rabbit Polyclonal Antibody
SKU:
CAB13410
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-213 of human CHMP2B (NP_054762.2).
Cytoplasm, Late endosome membrane, Peripheral membrane protein, cytosol.
Calculated MW:
24kDa
Observed MW:
28kDa
This gene encodes a component of the heteromeric ESCRT-III complex (Endosomal Sorting Complex Required for Transport III) that functions in the recycling or degradation of cell surface receptors. ESCRT-III functions in the concentration and invagination of ubiquitinated endosomal cargos into intralumenal vesicles. The protein encoded by this gene is found as a monomer in the cytosol or as an oligomer in ESCRT-III complexes on endosomal membranes. It is expressed in neurons of all major regions of the brain. Mutations in this gene result in one form of familial frontotemporal lobar degeneration.
Purification Method:
Affinity purification
Gene ID:
25978
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using CHMP2B antibody (CAB13410) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
