The CHK2 Rabbit Monoclonal Antibody (CAB19543) is a high-quality antibody specifically designed for research involving the CHK2 protein, a key player in cell cycle checkpoints and DNA damage response pathways. This antibody, produced in rabbits, is highly selective and sensitive for detecting CHK2 in human samples, making it a valuable tool for studies in oncology, genetics, and molecular biology.CHK2 is a tumor suppressor protein that is activated in response to DNA damage, leading to cell cycle arrest and DNA repair.
Dysregulation of CHK2 has been implicated in various cancers and genetic disorders, making it a target of interest for researchers studying tumorigenesis and genomic stability. The CHK2 Rabbit Monoclonal Antibody allows for precise detection and quantification of CHK2 protein levels in different cell types and experimental conditions, providing valuable insights into its role in cellular pathways and disease development.
Product Name:
Chk2 Rabbit Monoclonal Antibody
SKU:
CAB19543
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Chk2 (O96017).
In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Several transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
11200
Clone Number:
ARC57076
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using Chk2 Rabbit mAb (CAB19543) at 1:7000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
