The CHK1 Polyclonal Antibody (CAB20015) is a valuable tool for researchers studying CHK1, a key regulator of cell cycle checkpoints and DNA damage response. This antibody, produced in rabbits, is specifically designed for use in Western blotting and immunohistochemistry applications, providing reliable and accurate detection of CHK1 protein levels in human samples.CHK1 is a crucial protein in maintaining genomic stability and cell survival in response to DNA damage, making it a popular target in cancer research and drug development. Its role in cell cycle regulation and DNA repair pathways has garnered significant interest in understanding its function in tumorigenesis and potential therapeutic interventions.
By using the CHK1 Polyclonal Antibody, researchers can investigate the expression and activation of CHK1 in various cell types and experimental conditions, aiding in the elucidation of CHK1 signaling pathways and its implications in cancer biology. This antibody is a valuable asset for studies aiming to uncover the mechanisms underlying CHK1's role in cell cycle control and DNA repair processes.
Product Name:
Chk1 Polyclonal Antibody
SKU:
CAB21348
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 148-307 of Chk1 (NP_001265.2).
The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
1111
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates, using Chk1 Rabbit pAb (CAB21348) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer:3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: Q60s.
