Chk1 Polyclonal Antibody (CAB21348)
- SKU:
- CAB21348
- Product Type:
- Antibody
- Antibody Type:
- Polyclonal Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- WB
Frequently bought together:
Description
| Product Name: | Chk1 Polyclonal Antibody |
| SKU: | CAB21348 |
| Size: | 20uL, 100uL |
| Isotype: | IgG |
| Host Species: | Rabbit |
| Reactivity: | Human |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 148-307 of Chk1 (NP_001265.2). |
| Sequence: | DFGL ATVF RYNN RERL LNKM CGTL PYVA PELL KRRE FHAE PVDV WSCG IVLT AMLA GELP WDQP SDSC QEYS DWKE KKTY LNPW KKID SAPL ALLH KILV ENPS ARIT IPDI KKDR WYNK PLKK GAKR PRVT SGGV SESP SGFS KHIQ SNLD FSPV NSAS |
| Tested Applications: | WB ELISA |
| Recommended Dilution: | WB,1:500 - 1:2000 |
| Synonyms: | CHK1; Chk1 |
| Positive Sample: | A-431,293T |
| Conjugate: | Unconjugated |
| Cellular Localization: | Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center. |
| Calculated MW: | 54kDa |
| Observed MW: | 44kDa |
The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene.
| Purification Method: | Affinity purification |
| Gene ID: | 1111 |
| Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3. |
 | Western blot analysis of various lysates, using Chk1 Rabbit pAb (CAB21348) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer:3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: Q60s. |
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