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Chk1 Monoclonal Antibody (CAB22055)

SKU:
CAB22055
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Applications:
WB
€139 - €529
Frequently bought together:

Description

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Product Name:Chk1 Monoclonal Antibody
SKU:CAB22055
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 250-350 of human Chk1 (NP_001107593.1).
Sequence:PSAR ITIP DIKK DRWY NKPL KKGA KRPR VTSG GVSE SPSG FSKH IQSN LDFS PVNS ASSE ENVK YSSS QPEP RTGL SLWD TSPS YIDK LVQG ISFS QPTC P
Tested Applications:WB ELISA
Recommended Dilution:WB,1:2000 - 1:20000
Synonyms:CHK1; Chk1
Positive Sample:K-562
Conjugate:Unconjugated
Cellular Localization:centrosome, condensed nuclear chromosome, cytoplasm, cytosol, extracellular space, nucleoplasm, nucleus
Calculated MW:54kDa
Observed MW:56kDa

The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene.

Purification Method:Affinity purification
Gene ID:1111
Clone Number:ARC52506
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from K-562 cells, using Chk1 Rabbit mAb (CAB22055) at1:17000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 60s.

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