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Chicken Vasoactive Intestinal Peptide (VIP) ELISA Kit

SKU:
CHDL00055
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P48143
Sensitivity:
1.8pg/mL
Range:
7.812-500pg/mL
ELISA Type:
Sandwich
Synonyms:
PHM27,Intestinal peptide PHV-42,Peptide histidine valine 42,Peptide histidine methioninamide 27
Reactivity:
Chicken
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Chicken Vasoactive Intestinal Peptide (VIP) ELISA Kit

The Chicken Vasoactive Intestinal Peptide (VIP) ELISA Kit is specifically designed for the accurate detection of VIP levels in chicken serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.VIP is a neuropeptide that plays a vital role in the regulation of various physiological processes, including circadian rhythms, immune responses, and smooth muscle relaxation.

It is also involved in conditions such as inflammation, autoimmune diseases, and neurodegenerative disorders, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.Overall, the Chicken VIP ELISA Kit is a powerful tool for researchers seeking to investigate the role of VIP in chicken physiology and pathology, providing accurate and quantitative measurements for insightful data analysis.

Product Name:Chicken Vasoactive Intestinal Peptide (VIP) ELISA Kit
Product Code:CHDL00055
Size:96 Assays
Target:VIP
Synonyms:PHM27, Intestinal peptide PHV-42, Peptide histidine valine 42, Peptide histidine methioninamide 27
Detection Method:Sandwich
Range:7.812-500pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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