The Chicken TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit is a specialized assay designed for the quantitative measurement of TNF-α levels in chicken biological samples. TNF-α is a crucial pro-inflammatory cytokine that plays a pivotal role in various cellular functions, such as immune responses, inflammation, and apoptosis. This ELISA kit enables researchers to accurately quantify TNF-α concentrations in chicken samples, providing valuable insights into inflammation, immune regulation, and disease pathogenesis. Monitoring TNF-α levels is essential for understanding its impact on inflammatory processes, immune modulation, and the development of potential therapeutic strategies.
With its exceptional sensitivity and specificity, the Chicken TNF-α ELISA Kit ensures precise and reproducible results, allowing researchers to investigate the role of TNF-α in chicken physiology and pathology. Manufactured under stringent quality control standards, this kit offers reliable performance and ease of use, making it an excellent tool for research in chicken immunology and inflammation studies.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Chicken TNF-α. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Chicken TNF-α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Chicken TNF-α, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Chicken TNF-α. You can calculate the concentration of Chicken TNF-α in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-96
87-98
99-111
Average (%)
90
93
105
1:4
Range (%)
91-104
86-97
83-94
Average (%)
99
92
89
1:8
Range (%)
90-104
81-94
86-98
Average (%)
96
86
93
1:16
Range (%)
91-103
84-98
86-98
Average (%)
96
90
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-102
96
EDTA plasma (n=5)
85-101
92
Cell culture media (n=5)
85-97
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
110.23
215.17
685.07
113.81
206.36
631.63
Standard deviation
6.14
11.27
24.94
6.7
11.78
25.58
C V (%)
5.57
5.24
3.64
5.89
5.71
4.05
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100 μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Chicken TNF-α concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Chicken TNF-α in samples. No significant cross-reactivity or interference between Chicken TNF-α and analogues was observed.
