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Chicken Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) ELISA Kit

SKU:
CHDL00052
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P26652
Sensitivity:
0.076ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
SFD,TIMP Metallopeptidase Inhibitor 3,Metallopeptidase Inhibitor 3,Sorsby Fundus Dystrophy,Pseudoinflammatory
Reactivity:
Chicken
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
$779
Frequently bought together:

Description

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Chicken Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) ELISA Kit

The Chicken Tissue Inhibitors of Metalloproteinase-3 (TIMP3) ELISA Kit is a valuable tool for researchers studying the role of TIMP3 in various biological processes. This kit allows for the precise measurement of TIMP3 levels in chicken tissue samples, providing accurate and reliable results.TIMP3 is a key regulator of matrix metalloproteinases (MMPs), enzymes involved in tissue remodeling and extracellular matrix degradation. Dysregulation of TIMP3 has been implicated in various diseases, including cancer, cardiovascular diseases, and inflammation.

By measuring TIMP3 levels, researchers can gain insight into the mechanisms underlying these conditions and identify potential therapeutic targets.The Chicken TIMP3 ELISA Kit offers high sensitivity and specificity, allowing for the detection of TIMP3 in a variety of sample types, including serum, plasma, and tissue lysates. With its ease of use and robust performance, this kit is a valuable tool for investigating the role of TIMP3 in chicken physiology and pathology.

Product Name:Chicken Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) ELISA Kit
Product Code:CHDL00052
Size:96 Assays
Target:TIMP3
Synonyms:SFD, TIMP Metallopeptidase Inhibitor 3, Metallopeptidase Inhibitor 3, Sorsby Fundus Dystrophy, Pseudoinflammatory
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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