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Chicken Secretory Immunoglobulin A (sIgA) ELISA Kit

SKU:
CHDL00050
Product Type:
ELISA Kit
Sensitivity:
0.073ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
S-IgA
Reactivity:
Chicken
Sample Type:
Serum, Plasma And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Chicken Secretory Immunoglobulin A (sIgA) ELISA Kit

The Chicken Secretory Immunoglobulin A (sIgA) ELISA Kit is a highly accurate and reliable tool for detecting levels of secretory immunoglobulin A in chicken serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.Secretory immunoglobulin A plays a crucial role in the immune system of chickens, providing protection against infections and pathogens in mucosal surfaces such as the respiratory and digestive tracts.

Monitoring sIgA levels can help researchers better understand the immune response in chickens and develop strategies for improving immune health and disease resistance in poultry.Overall, the Chicken Secretory Immunoglobulin A (sIgA) ELISA Kit is an essential tool for studying avian immunology, infectious diseases, and vaccine development in chicken populations, offering valuable insights into the immune status and health of poultry.

Product Name:Chicken Secretory Immunoglobulin A (sIgA) ELISA Kit
Product Code:CHDL00050
Size:96 Assays
Target:sIgA
Synonyms:S-IgA
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)85-10092
EDTA plasma (n=5)85-10394
Heparin plasma (n=5)87-9993
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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