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Chicken Nerve Growth Factor (NGF) ELISA Kit

SKU:
CHDL00046
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P05200
Sensitivity:
10.6pg/mL
Range:
31.25-2000pg/mL
ELISA Type:
Sandwich
Synonyms:
NGFB,Beta-NGF,HSAN5,NGF-B,Beta-Nerve Growth Factor
Reactivity:
Chicken
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Chicken Nerve Growth Factor (NGF) ELISA Kit

The Chicken Nerve Growth Factor (NGF) ELISA Kit is specifically designed for the precise measurement of NGF levels in chicken serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results for various research purposes.NGF is a vital protein responsible for promoting the growth and survival of nerve cells. It plays a crucial role in the development and maintenance of the nervous system, making it a key factor in various neurological disorders and conditions.

By studying NGF levels, researchers can gain valuable insights into nerve regeneration, neurodevelopmental disorders, and potential therapeutic interventions.Overall, the Chicken NGF ELISA Kit is a valuable tool for researchers interested in studying nerve growth factors and their implications in neurological health and disease. Its reliability and accuracy make it an excellent choice for a wide range of research applications.

Product Name:Chicken Nerve Growth Factor (NGF) ELISA Kit
Product Code:CHDL00046
Size:96 Assays
Target:NGF
Synonyms:NGFB, Beta-NGF, HSAN5, NGF-B, Beta-Nerve Growth Factor
Detection Method:Sandwich
Range:31.25-2000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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