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Chicken Macrophage Inflammatory Protein 1 Beta (MIP1b) ELISA Kit

SKU:
CHDL00042
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q90826
Sensitivity:
25.8pg/mL
Range:
62.5-4000pg/mL
ELISA Type:
Sandwich
Synonyms:
CCL4,ACT2,G-26,LAG1,MIP1-B,SCYA4,Chemokine C-C-Motif Ligand 4,Small Inducible Cytokine A4,Homologous To Mouse Mip-1b
Reactivity:
Chicken
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Chicken Macrophage Inflammatory Protein 1 Beta (MIP1b) ELISA Kit

The Chicken Macrophage Inflammatory Protein-1 Beta (MIP1B) ELISA Kit is a powerful tool for the accurate measurement of MIP1B levels in chicken samples including serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit ensures reliable and reproducible results for a variety of research applications.MIP1B is an important chemokine involved in immune responses, specifically in recruiting and activating immune cells such as macrophages.

It plays a crucial role in inflammatory processes and has been implicated in various diseases and conditions, making it a valuable biomarker for studying immune responses and potential therapeutic interventions.Overall, the Chicken MIP1B ELISA Kit offers researchers a reliable and efficient method for quantifying MIP1B levels in chicken samples, providing valuable insights into immune system function and inflammatory pathways.

Product Name:Chicken Macrophage Inflammatory Protein 1 Beta (MIP1b) ELISA Kit
Product Code:CHDL00042
Size:96 Assays
Target:MIP1b
Synonyms:CCL4, ACT2, G-26, LAG1, MIP1-B, SCYA4, Chemokine C-C-Motif Ligand 4, Small Inducible Cytokine A4, Homologous To Mouse Mip-1b
Detection Method:Sandwich
Range:62.5-4,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)89-10295
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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