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Chicken Interleukin 2 (IL-2) ELISA Kit

SKU:
CHDL00035
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
O42288
Sensitivity:
6.5pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
TCGF,Lymphokine,T-Cell Growth Factor,Aldesleukin
Reactivity:
Chicken
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Chicken Interleukin 2 (IL-2) ELISA Kit

The Chicken Interleukin-2 (IL-2) ELISA Kit from Assay Genie is a highly reliable and accurate tool for detecting IL-2 levels in chicken serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures precise and reproducible results, making it a valuable asset for a variety of research applications.Interleukin-2 is a critical protein involved in regulating immune responses and promoting T cell growth and differentiation. Its levels can serve as important indicators of immune system activity and dysfunction, making it a key biomarker in studying immune-related diseases and treatments.

With the Chicken Interleukin-2 (IL-2) ELISA Kit, researchers can confidently measure and analyze IL-2 levels in chicken samples, furthering their understanding of immune responses and paving the way for potential therapeutic advancements. Partner with Assay Genie for dependable and accurate IL-2 detection in your research endeavors.

Product Name:Chicken Interleukin 2 (IL-2) ELISA Kit
Product Code:CHDL00035
Size:96 Assays
Target:IL2
Synonyms:TCGF, Lymphokine, T-Cell Growth Factor, Aldesleukin
Detection Method:Sandwich
Range:15.625-1,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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