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Chicken Interleukin 18 (IL-18) ELISA Kit

SKU:
CHDL00033
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q8QFQ8
Sensitivity:
7.3pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
IGIF,IL-1g,IL1F4,IL1-F4,Interferon-Gamma-Inducing Factor,Interleukin-1 Family Member 4,Iboctadekin,Interleukin-1 Gamma
Reactivity:
Chicken
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Chicken Interleukin 18 (IL-18) ELISA Kit

The Chicken Interleukin-18 (IL18) ELISA Kit is a specialized assay designed for the accurate detection of interleukin-18 levels in chicken serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results that are essential for various research applications.Interleukin-18 is a key cytokine that plays a crucial role in the immune response, inflammation, and autoimmune diseases in chickens.

It also aids in the regulation of innate and adaptive immunity. Monitoring IL18 levels can provide valuable insights into the immune system functioning and disease progression in poultry.Overall, the Chicken Interleukin-18 (IL18) ELISA Kit is an invaluable tool for researchers studying the immune response and inflammatory processes in chickens, making it an essential component for poultry research and veterinary studies.

Product Name:Chicken Interleukin 18 (IL-18) ELISA Kit
Product Code:CHDL00033
Size:96 Assays
Target:IL18
Synonyms:IGIF, IL-1g, IL1F4, IL1-F4, Interferon-Gamma-Inducing Factor, Interleukin-1 Family Member 4, Iboctadekin, Interleukin-1 Gamma
Detection Method:Sandwich
Range:15.625-1000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)89-10295
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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