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Chicken Interleukin 12A (IL-12A) ELISA Kit

SKU:
CHDL00029
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q6X0L0
Sensitivity:
7.2pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
p35,IL12-A,CLMF1,NFSK,NKSF1,Natural Killer Cell Stimulatory Factor 1,Cytotoxic Lymphocyte Maturation Factor 1,NF Cell Stimulatory Factor Chain 1
Reactivity:
Chicken
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Chicken Interleukin 12A (IL-12A) ELISA Kit

The Chicken Interleukin-12A (IL12A) ELISA Kit is a reliable tool for the accurate detection of interleukin-12A levels in chicken samples, including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.Interleukin-12A is a crucial cytokine involved in the regulation of immune responses, particularly in the activation of T cells and natural killer cells. It plays a key role in the immune system's defense against pathogens and is essential for maintaining immune homeostasis.

The Chicken Interleukin-12A (IL12A) ELISA Kit is an invaluable resource for studying the immune response in chickens, as well as for investigating the pathogenesis of infectious diseases and developing potential immunotherapies. With its reliable performance and user-friendly protocol, this kit is a must-have for researchers working in the field of poultry immunology.

Product Name:Chicken Interleukin 12A (IL-12A) ELISA Kit
Product Code:CHDL00029
Size:96 Assays
Target:IL12A
Synonyms:p35, IL12-A, CLMF1, NFSK, NKSF1, Natural Killer Cell Stimulatory Factor 1, Cytotoxic Lymphocyte Maturation Factor 1, NF Cell Stimulatory Factor Chain 1
Detection Method:Sandwich
Range:15.625-1000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)90-9994
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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