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Chicken Interferon Alpha (IFNa) ELISA Kit

SKU:
CHDL00023
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P42165
Sensitivity:
28pg/mL
Range:
78.125-5000pg/mL
ELISA Type:
Sandwich
Synonyms:
IFNA1,IFL,LeIF D,IFN,IFN-Alpha,IFNA13,IFN-A,IFNAP22,IFN-Alpha 1b,Interferon Alpha 1b,Interferon, Leukocytic,IFN, Leukocyte,Interferon alpha-D
Reactivity:
Chicken
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Chicken Interferon Alpha (IFNa) ELISA Kit

The Chicken Interferon Alpha (IFNα) ELISA Kit is a reliable and efficient tool for the accurate detection of interferon alpha levels in chicken serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures consistent and precise results, making it an ideal choice for a variety of research applications.Interferon alpha is a key player in the immune response, playing a critical role in antiviral defense and immune regulation. By measuring interferon alpha levels, researchers can gain valuable insights into the immune response in chickens, which can be beneficial for studying infectious diseases, vaccine development, and immune system disorders in poultry.

This Chicken Interferon Alpha (IFNα) ELISA Kit offers a user-friendly protocol and delivers reliable data, making it a valuable tool for researchers looking to explore the immune response in chickens and develop novel strategies for disease control and prevention in poultry.

Product Name:Chicken Interferon Alpha (IFNa) ELISA Kit
Product Code:CHDL00023
Size:96 Assays
Target:IFNa
Synonyms:IFNA1, IFL, LeIF D, IFN, IFN-Alpha, IFNA13, IFN-A, IFNAP22, IFN-Alpha 1b, Interferon Alpha 1b, Interferon, Leukocytic, IFN, Leukocyte, Interferon alpha-D
Detection Method:Sandwich
Range:78.125-5000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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