Chicken CCL20 DIY ELISA Kit
- SKU:
- KES0221
- Product Type:
- ELISA Kit
- Reactivity:
- Chicken
- Applications:
- ELISA
- ELISA Type:
- DIY ELISA
- Size:
- 10 Plates
- Synonyms:
- CCL20, CKb4, Exodus, LARC, MIP-3-alpha, MIP-3a, MIP3A, SCYA20, ST38
Description
Chicken CCL20 DIY ELISA Kit
The Chicken CCL20 ELISA Kit is specifically designed for the precise measurement of CCL20 levels in chicken serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing consistent and accurate results, making it an invaluable tool for various research applications.CCL20, also known as macrophage inflammatory protein-3 alpha (MIP-3 alpha), plays a critical role in the immune system by attracting immune cells to sites of inflammation and infection.
It is involved in regulating immune responses, making it a key biomarker for studying inflammatory conditions and immune-related diseases in chickens.With the Chicken CCL20 ELISA Kit, researchers can gain valuable insights into the immune response in chickens and investigate the potential role of CCL20 in various poultry diseases and infections. This kit provides a reliable and efficient method for quantifying CCL20 levels, facilitating innovative research and advancements in poultry health.
| Product Name: | Chicken CCL20 DIY ELISA |
| Product Code: | KES0221 |
| Species: | Chicken |
| Target: | CCL20 |
| Synonyms: | CCL20, CKb4, Exodus, LARC, MIP-3-alpha, MIP-3a, MIP3A, SCYA20, ST38 |
| Application: | ELISA |
| ELISA Type: | DIY ELISA Kit |
| Size: | 10 Plates |
| Shipping: | Room temperature |
| Storage: | Stable for up to twelve months from date of receipt at 2-8°C. |
| Description | Usage | Quantity |
| Anti-Chicken CCL20 Polyclonal Antibody | Capture Antibody | 100 µg |
| Biotinylated Anti-Chicken CCL20 Polyclonal Antibody | Detection Antibody | 50 µg |
| Chicken CCL20 Recombinant Protein | Standard | 5 µg |
| Reagent | Suggested Formulation |
| DPBS: | 0.008M sodium phosphate, 0.002M potassium phosphate, 0.14M sodium chloride, 0.01M potassium chloride, pH 7.4 |
| 96-well ELISA Plate: | Clear, flat-bottom, high-binding 96-well plate, 8-wells per strip, 350 µL per well (ELISA Plates: KESAP003) |
| Standard and Sample Diluent: | The optimal Standard and Sample Diluent will need to be determined for each sample type to obtain optimal recovery and linearity. The appropriate Standard and Sample Diluent will mimic the sample's response to a known quantity of protein standard and will provide linear results when diluted. Often a 1:4 dilution of the sample in Reagent Diluent will provide acceptable recovery and linearity. |
| Reagent Diluent and Blocking Buffer: | 4% BSA in DPBS, 0.2 µm filtered |
| Wash Buffer: | 0.05% Tween®-20 in DPBS |
| Streptavidin-HRP: | Enzymatic reagent to react with biotinylated detection antibody (Streptavidin-HRP: KESAP002) |
| Substrate: | 3,3',5,5'-tetramethylbenzidine (TMB) Substrate (ELISA Accessory Pack: KESAP001) |
| Stop Solution: | 0.18 M Sulfuric Acid (ELISA Accessory Pack: KESAP001) |
| Plate Sealer: | Adhesive film to prevent evaporation |
*Note: Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
| Step | Procedure |
| 1. | Prepare Capture Antibody in DPBS at desired working concentration. |
| 2. | Add 100 µL of Capture Antibody Working Solution to appropriate wells. |
| 3. | Cover plate with Plate Sealer and incubate at room temperature (20-25°C) for 12-24 hours. |
| 4. | Empty Capture Antibody Working Solution from plate. Blot plate onto paper towels or other absorbent material. |
| 5. | Add 100 µL of Blocking Buffer to appropriate wells. |
| 6. | Cover plate with Plate Sealer and incubate at room temperature for 1-3 hours. |
| 7. | Empty Blocking Buffer from plate. Blot plate onto paper towels or other absorbent material. |
| 8. | Prepare Standard and sample as desired with Standard and Sample Diluent. |
| 9. | Add 100 µL of Standard or sample to appropriate wells. Note: Run each Standard or sample in duplicate. |
| 10. | Cover plate with Plate Sealer and incubate at room temperature for 1 hour. |
| 11. | Wash plate FOUR times with Wash Buffer. Note: Gently squeeze the long sides of plate frame before washing to ensure all strips remain securely in the frame. Empty plate contents. Use a squirt wash bottle to vigorously fill each well completely with 1X Wash Buffer, then empty plate contents. Repeat procedure three additional times for a total of FOUR washes. Blot plate onto paper towels or other absorbent material. |
| 12. | Prepare Detection Antibody in Reagent Diluent at desired working concentration. |
| 13. | Add 100 µL of Detection Antibody Working Solution to each well. |
| 14. | Cover plate with Plate Sealer and incubate at room temperature for 1 hour. |
| 15. | Wash plate FOUR times with Wash Buffer as described in step 11. |
| 16. | Prepare Streptavidin-HRP in Reagent Diluent at desired working concentration. |
| 17. | Add 100 µL of Streptavidin-HRP Working Solution to each well. |
| 18. | Cover plate with Plate Sealer and incubate at room temperature for 30 minutes. |
| 19. | Wash plate FOUR times with Wash Buffer as described in step 11. |
| 20. | Add 100 µL of TMB Substrate Solution to each well. |
| 21. | Develop the plate in the dark at room temperature for 30 minutes or as desired. Note: Do NOT cover plate with Plate Sealer. |
| 22. | Stop reaction by adding 100 µL of Stop Solution to each well. |
| 23. | Measure absorbance on a plate reader at 450 nm. |
The Chicken CCL20 DIY ELISA kit from Assay Genie can assay for CCL20 in the following samples: serum, blood, plasma, cell culture supernatant and other related supernatants and tissues. Chicken CCL20 DIY ELISA Kit from Assay Genie allows researchers to develop their own ELISA plates for CCL20 using our unique combination of capture and detection antibodies.
Each Chicken CCL20 DIY ELISA Kit contains capture antibody, standard, and detection antibody for development of an CCL20 ELISA. CCL20 antibodies in this kit have been determined to function in an ELISA with the CCL20 standard provided. Optimal buffers, concentrations, incubation times, incubation temperatures, and methods for the ELISA have not been determined and require optimisation for the development of this kit. A working knowledge of ELISA is strongly recommended.
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