The CHGA Polyclonal Antibody (PAC020910) is a valuable tool for researchers studying Chromogranin A (CHGA), a protein involved in the regulation of neuroendocrine function and stress response. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in various applications, including Western blotting.CHGA is a key player in the stress response pathway, functioning to modulate the release of hormones and neurotransmitters in times of stress. Dysregulation of CHGA has been implicated in a variety of diseases, including cardiovascular disorders and neurodegenerative conditions.
By targeting CHGA with this antibody, researchers can further investigate its role in these diseases and potentially uncover new therapeutic targets.Overall, the CHGA Polyclonal Antibody is a versatile tool for researchers interested in delving deeper into the functions of Chromogranin A and its implications in various disease processes. Its high specificity and sensitivity make it an ideal choice for studies in neuroendocrinology, cardiovascular research, and stress-related disorders.
Gel: 8%SDS-PAGE,Lysate: 40 µg,Lane 1-4: A549 cells, 293T cells, Human normal kidney tissue, Hela cells,Primary antibody: PACO20910(CHGA Antibody) at dilution 1/200 dilution,Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,Exposure time: 1 minute.
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using PACO20910(CHGA Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200).
Background:
The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells. This gene product is a precursor to three biologically active peptides; vasostatin, pancreastatin, and parastatin. These peptides act as autocrine or paracrine negative modulators of the neuroendocrine system. Two other peptides, catestatin and chromofungin, have antimicrobial activity and antifungal activity, respectively. Two transcript variants encoding different isoforms have been found for this gene.
Synonyms:
chromogranin A (parathyroid secretory protein 1)
UniProt Protein Function:
CHGA: a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells and is a precursor to multiple biologically active peptides including vasostatin, pancreastatin, and chromostatin. Binds calcium with a low-affinity. Vasostatin negatively regulates angiogenesis and has antibacterial activity against some Gram-positive bacteria. Pancreastatin inhibits glucose induced insulin release from the pancreas. Chromostatin inhibits catecholamine release from chromaffin cells.
UniProt Protein Details:
Protein type:Secreted, signal peptide; Secreted; Vesicle; Cell adhesion
Chromosomal Location of Human Ortholog: 14q32
Cellular Component: extracellular space; perinuclear region of cytoplasm; secretory granule
Biological Process: defense response to Gram-negative bacterium; defense response to Gram-positive bacterium; mast cell activation; mast cell chemotaxis; mast cell cytokine production; mast cell degranulation; negative regulation of catecholamine secretion; negative regulation of vasodilation; organelle organization and biogenesis; positive regulation of cAMP metabolic process; regulation of blood pressure; regulation of the force of heart contraction
NCBI Summary:
The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells. This gene product is a precursor to three biologically active peptides; vasostatin, pancreastatin, and parastatin. These peptides act as autocrine or paracrine negative modulators of the neuroendocrine system. Two other peptides, catestatin and chromofungin, have antimicrobial activity and antifungal activity, respectively. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]
