The CHFR Polyclonal Antibody (CAB10447) is a valuable tool for researchers studying the role of CHFR in cell cycle regulation and tumor suppression. CHFR, a key player in the mitotic checkpoint control pathway, is essential for maintaining genomic stability and preventing the formation of abnormal cells.This polyclonal antibody, raised in rabbits, exhibits high reactivity with human samples and is validated for use in various applications, including Western blot and immunofluorescence. By binding to the CHFR protein, this antibody allows for the detection and analysis of CHFR expression in different cell types, providing valuable insights into its functions in physiological and pathological contexts.
Given the essential role of CHFR in controlling cell division and preventing the development of cancerous cells, understanding its mechanisms of action can aid in the development of novel therapeutic strategies for cancer treatment. This antibody is an indispensable tool for researchers investigating CHFR's involvement in tumorigenesis and its potential as a target for anti-cancer therapies.
Product Name:
CHFR Rabbit Polyclonal Antibody
SKU:
CAB10447
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human CHFR (NP_001154818.1).
This gene encodes an E3 ubiquitin-protein ligase required for the maintenance of the antephase checkpoint that regulates cell cycle entry into mitosis and, therefore, may play a key role in cell cycle progression and tumorigenesis. The encoded protein has an N-terminal forkhead-associated domain, a central RING-finger domain, and a cysteine-rich C-terminal region. Alternatively spliced transcript variants that encode different protein isoforms have been described.
Purification Method:
Affinity purification
Gene ID:
55743
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using CHFR Rabbit pAb (CAB10447) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
