The CHEK2 Polyclonal Antibody (CAB0466) is a valuable tool for researchers studying the CHEK2 protein, an important player in the DNA damage response pathway. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blotting applications. By binding to the CHEK2 protein, this antibody allows for precise detection and analysis of this key regulator of cell cycle checkpoints and DNA repair mechanisms.CHEK2, also known as checkpoint kinase 2, plays a crucial role in maintaining genomic stability and preventing the accumulation of DNA damage that can lead to cancer development.
Mutations in the CHEK2 gene have been associated with an increased risk of certain cancers, making it an attractive target for cancer research and personalized medicine approaches. By studying the function and regulation of CHEK2, researchers can gain valuable insights into the mechanisms underlying tumorigenesis and identify potential therapeutic targets for cancer treatment.
Product Name:
Chk2 Rabbit Polyclonal Antibody
SKU:
CAB0466
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human Chk2 (NP_009125.1).
In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Several transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
11200
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using Chk2 Rabbit pAb (CAB0466) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
