Chk2 Rabbit Polyclonal Antibody (CAB0466)
- SKU:
- CAB0466
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Death
Description
Product Name: | Chk2 Rabbit Polyclonal Antibody |
SKU: | CAB0466 |
Size: | 20uL, 100uL |
Isotype: | IgG |
Host Species: | Rabbit |
Reactivity: | Human |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human Chk2 (NP_009125.1). |
Sequence: | MSRE SDVE AQQS HGSS ACSQ PHGS VTQS QGSS SQSQ GISS SSTS TMPN SSQS SHSS SGTL SSLE TVST QELY SIPE DQEP EDQE PEEP TPAP WARL WALQ DGFA NLEC VNDN YWFG RDKS CEYC FDEP LLKR TDKY RTYS KKHF RIFR EVGP KNSY IAYI EDHS GNGT FVNT ELVG KGKR RPLN NNSE IALS LSRN KVFV FFDL TVDD QSVY PKAL RDEY |
Tested Applications: | WB IF/ICC IP ELISA |
Recommended Dilution: | WB,1:100 - 1:500 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells |
Synonyms: | CDS1; CHK2; LFS2; RAD53; hCds1; HuCds1; PP1425; Chk2 |
Positive Sample: | HeLa,Jurkat |
Conjugate: | Unconjugated |
Cellular Localization: | Nucleus, Nucleus, PML body, nucleoplasm. |
Calculated MW: | 61kDa |
Observed MW: | 65kDa |
In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Several transcript variants encoding different isoforms have been found for this gene.
Purification Method: | Affinity purification |
Gene ID: | 11200 |
Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. |