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Chk1 Rabbit Polyclonal Antibody (CAB7653)

SKU:
CAB7653
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle
  • Cell Cycle Antibodies 2 Anti-Chk1 Antibody CAB7653
  • Cell Cycle Antibodies 2 Anti-Chk1 Antibody CAB7653
  • Cell Cycle Antibodies 2 Anti-Chk1 Antibody CAB7653
  • Cell Cycle Antibodies 2 Anti-Chk1 Antibody CAB7653
  • Cell Cycle Antibodies 2 Anti-Chk1 Antibody CAB7653
€139 - €419
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Chk1 Rabbit Polyclonal Antibody
SKU:CAB7653
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 148-307 of Chk1 (NP_001265.2).
Sequence:DFGL ATVF RYNN RERL LNKM CGTL PYVA PELL KRRE FHAE PVDV WSCG IVLT AMLA GELP WDQP SDSC QEYS DWKE KKTY LNPW KKID SAPL ALLH KILV ENPS ARIT IPDI KKDR WYNK PLKK GAKR PRVT SGGV SESP SGFS KHIQ SNLD FSPV NSAS
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200
Synonyms:CHK1; Chk1
Positive Sample:HT-29,NIH/3T3,Mouse testis,Rat testis
Conjugate:Unconjugated
Cellular Localization:Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center.
Calculated MW:54kDa
Observed MW:56kDa

The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene.

Purification Method:Affinity purification
Gene ID:1111
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using Chk1 Rabbit pAb (CAB7653) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

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