The CHEK1 Polyclonal Antibody (CAB7653) is a valuable tool for researchers studying the protein CHEK1, a key player in the DNA damage response pathway. This antibody, generated in rabbits, exhibits high specificity and sensitivity for detecting CHEK1 in human samples, making it ideal for use in Western blot applications.CHEK1 is a critical kinase involved in cell cycle checkpoint control and DNA repair mechanisms. Dysregulation of CHEK1 has been linked to various diseases, including cancer, making it a promising target for therapeutic interventions.
The use of the CHEK1 Polyclonal Antibody allows for the accurate detection and quantification of CHEK1 levels in different cell types, enabling researchers to further explore its role in cancer biology and develop targeted therapies for cancer treatment.With its reliable performance and compatibility with various experimental settings, the CHEK1 Polyclonal Antibody is a valuable tool for scientists investigating the function and regulation of CHEK1 in cancer and other related research fields.
Product Name:
Chk1 Rabbit Polyclonal Antibody
SKU:
CAB7653
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 148-307 of Chk1 (NP_001265.2).
The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
1111
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates using Chk1 Rabbit pAb (CAB7653) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
