The CHD3 Polyclonal Antibody (CAB2221) is a valuable tool for researchers studying the CHD3 protein, which is involved in chromatin remodeling and gene expression regulation. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications.CHD3, also known as Mi-2α, is a member of the CHD (chromodomain helicase DNA-binding) family of proteins and plays a crucial role in epigenetic regulation. It is involved in maintaining chromatin structure and promoting gene silencing, making it essential for cellular development and differentiation.
By utilizing the CHD3 Polyclonal Antibody, researchers can detect and analyze the expression levels of CHD3 in various cell types, providing valuable insights into its function and contribution to different biological processes. This antibody is particularly useful for studies in the fields of epigenetics, gene regulation, and cancer research. A deeper understanding of CHD3 and its mechanisms of action may lead to the development of novel therapeutic strategies targeting chromatin remodeling in disease conditions.
Product Name:
CHD3 Rabbit Polyclonal Antibody
SKU:
CAB2221
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1900-2000 of human CHD3 (NP_001005273.1).
This gene encodes a member of the CHD family of proteins which are characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. This protein is one of the components of a histone deacetylase complex referred to as the Mi-2/NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Chromatin remodeling is essential for many processes including transcription. Autoantibodies against this protein are found in a subset of patients with dermatomyositis. Three alternatively spliced transcripts encoding different isoforms have been described.
Purification Method:
Affinity purification
Gene ID:
1107
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using CHD3 antibody (CAB2221) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 30s.
