The CHCHD2 Polyclonal Antibody (CAB16645) is a valuable tool for researchers studying CHCHD2, a protein involved in regulating mitochondrial function and energy metabolism. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the CHCHD2 protein, this antibody enables accurate detection and analysis in various cell types, making it ideal for investigations in the fields of mitochondrial biology and neurodegenerative diseases.CHCHD2 is known to play a crucial role in maintaining mitochondrial integrity and function, making it a key target for research into conditions such as neurodegenerative disorders, metabolic diseases, and aging.
Understanding the mechanisms through which CHCHD2 influences mitochondrial health is essential for developing strategies to prevent or treat these debilitating conditions. The CHCHD2 Polyclonal Antibody provides researchers with a reliable tool to study the function and regulation of this important protein, advancing our knowledge of mitochondrial biology and its implications for human health.
Product Name:
[KO Validated] CHCHD2 Rabbit Polyclonal Antibody
SKU:
CAB16645
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 75-145 of human CHCHD2 (NP_057223.1).
The protein encoded by this gene belongs to a class of eukaryotic CX(9)C proteins characterized by four cysteine residues spaced ten amino acids apart from one another. These residues form disulfide linkages that define a CHCH fold. In response to stress, the protein translocates from the mitochondrial intermembrane space to the nucleus where it binds to a highly conserved 13 nucleotide oxygen responsive element in the promoter of cytochrome oxidase 4I2, a subunit of the terminal enzyme of the electron transport chain. In concert with recombination signal sequence-binding protein J, binding of this protein activates the oxygen responsive element at four percent oxygen. In addition, it has been shown that this protein is a negative regulator of mitochondria-mediated apoptosis. In response to apoptotic stimuli, mitochondrial levels of this protein decrease, allowing BCL2-associated X protein to oligomerize and activate the caspase cascade. Pseudogenes of this gene are found on multiple chromosomes. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
51142
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using [KO Validated] CHCHD2 Rabbit pAb (CAB16645) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
