The CH25H Polyclonal Antibody (PAC051418) is a valuable tool for researchers studying the CH25H protein, which is involved in cholesterol metabolism and immune regulation. This antibody, produced through rabbit immunization, exhibits high reactivity with human samples and is specifically validated for Western blot applications. By targeting the CH25H protein, researchers can detect and analyze its presence in various cell types, making it an ideal reagent for studies in immunology, lipid metabolism, and inflammation.CH25H, also known as cholesterol 25-hydroxylase, is a key enzyme in the conversion of cholesterol to 25-hydroxycholesterol, a molecule that modulates immune responses and inflammation.
Dysregulation of CH25H has been implicated in various diseases, including atherosclerosis, multiple sclerosis, and viral infections. By studying the functions and mechanisms of CH25H, researchers can gain valuable insights into the pathogenesis of these diseases and potentially develop targeted therapies.Overall, the CH25H Polyclonal Antibody is a reliable tool for investigating the role of CH25H in health and disease. Its specificity, sensitivity, and application in Western blotting make it a valuable asset for scientists working in the fields of immunology, lipid biology, and inflammatory disorders.
Antibody Name:
CH25H Antibody (PACO51418)
Antibody SKU:
PACO51418
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:10000, IHC:1:20-1:200
Species Reactivity:
Human
Immunogen:
Recombinant Human Cholesterol 25-hydroxylase protein (142-272AA)
Immunohistochemistry of paraffin-embedded human colon cancer using PACO51418 at dilution of 1:100.
Immunohistochemistry of paraffin-embedded human kidney tissue using PACO51418 at dilution of 1:100.
Background:
Catalyzes the formation of 25-hydroxycholesterol from cholesterol, leading to repress cholesterol biosynthetic enzymes. Plays a key role in cell positioning and movement in lymphoid tissues: 25-hydroxycholesterol is an intermediate in biosynthesis of 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC), an oxysterol that acts as a ligand for the G protein-coupled receptor GPR183/EBI2, a chemotactic receptor for a number of lymphoid cells. May play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. In testis, production of 25-hydroxycholesterol by macrophages may play a role in Leydig cell differentiation.
Catalyzes the formation of 25-hydroxycholesterol from cholesterol, leading to repress cholesterol biosynthetic enzymes (PubMed:9852097). Plays a key role in cell positioning and movement in lymphoid tissues: 25-hydroxycholesterol is an intermediate in biosynthesis of 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC), an oxysterol that acts as a ligand for the G protein-coupled receptor GPR183/EBI2, a chemotactic receptor for a number of lymphoid cells. May play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. In testis, production of 25-hydroxycholesterol by macrophages may play a role in Leydig cell differentiation.
UniProt Protein Details:
NCBI Summary:
This is an intronless gene that is involved in cholesterol and lipid metabolism. The encoded protein is a membrane protein and contains clusters of histidine residues essential for catalytic activity. Unlike most other sterol hydroxylases, this enzyme is a member of a small family of enzymes that utilize diiron cofactors to catalyze the hydroxylation of hydrophobic substrates. [provided by RefSeq, Jul 2008]
