The cGMP (Cyclic GMP) ELISA Kit from AssayGenie is a highly sensitive and specific assay designed for the accurate detection of cGMP levels in various samples including serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an essential tool for research applications in the fields of cardiovascular diseases, neurological disorders, and more.Cyclic GMP (cGMP) is a crucial signaling molecule involved in regulating various physiological processes, including smooth muscle relaxation, platelet aggregation, and neurotransmission.
Dysregulation of cGMP levels has been linked to conditions such as hypertension, erectile dysfunction, and heart failure, highlighting the importance of monitoring and studying this biomarker.With the AssayGenie cGMP ELISA Kit, researchers can accurately quantify cGMP levels in their samples, aiding in the understanding of disease mechanisms and the development of potential therapeutic interventions. Trust AssayGenie for reliable and high-quality ELISA kits for your research needs.
Product Name:
cGMP (Cyclic GMP) ELISA Kit (UNES00052)
SKU:
UNES00052
Target:
cGMP (Cyclic GMP) (UNES00052)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
0.47 pmol/mL
Detection range:
0.78-50 pmol/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with cGMP. During the reaction, cGMP in the sample or standard competes with a fixed amount of cGMP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to cGMP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of cGMP in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-104
88-100
89-102
Average (%)
96
94
94
1:4
Range (%)
94-107
88-101
89-101
Average (%)
101
94
95
1:8
Range (%)
96-111
90-106
89-102
Average (%)
102
97
97
1:16
Range (%)
94-108
92-103
90-104
Average (%)
102
97
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-104
96
EDTA plasma (n=5)
91-108
99
Cell culture media (n=5)
86-97
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
98.4
299.9
999.0
92.4
326.3
1055.7
Standard deviation
5.2
13.5
35.0
4.8
18.6
53.8
C V (%)
5.28
4.5
3.5
5.19
5.7
5.1
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of cGMP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes cGMP in samples. No significant cross-reactivity or interference between cGMP and analogues was observed.
