The CFL1/CFL2 Antibody (PACO22037) is a high-quality polyclonal antibody designed for research involving CFL1 and CFL2 proteins, key regulators of actin cytoskeleton dynamics in cells. This antibody, raised in rabbits, is highly specific and reactive with human, mouse, and rat samples, making it suitable for various experimental applications.CFL1 and CFL2 are essential proteins involved in actin filament severing and depolymerization processes, critical for cell migration, adhesion, and shape changes. Dysregulation of CFL proteins has been linked to various diseases, including cancer, neurodegenerative disorders, and autoimmune conditions.
Understanding the role of CFL1 and CFL2 in these pathological processes is crucial for developing targeted therapies.The CFL1/CFL2 Antibody enables the detection and analysis of CFL1 and CFL2 proteins in different cell types and tissues, making it an invaluable tool for researchers in cell biology, cancer biology, and neuroscience. Its validated use in Western blot applications ensures reliable results for studying the function and regulation of CFL proteins in health and disease.
Antibody Name:
CFL1/CFL2 Antibody (PACO22037)
Antibody SKU:
PACO22037
Size:
100ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:10000, WB:1:500-1:3000
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Synthesized peptide derived from internal of human Cofilin.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western blot analysis of extracts from Jurkat cells and HeLa cells, using Cofilin antibody.
Background:
Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity. Regulates actin cytoskeleton dynamics. Important for normal progress through mitosis and normal cytokinesis. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation.
Synonyms:
CFL2; COF2; Cofilin 2; Cofilin; muscle isoform
UniProt Protein Function:
Cofilin-1: a cytoskeletal protein that controls actin depolymerization. Has a 5-10-fold higher affinity for ATP-actin monomers than cofilin-1. May promote filament assembly rather than disassembly. Two alternatively spliced variants are described. Isoform b is expressed predominantly in skeletal muscle and heart, while isoform a is expressed in various tissues.
UniProt Protein Details:
Protein type:Motility/polarity/chemotaxis; Nuclear receptor co-regulator; Cytoskeletal
Biological Process: actin cytoskeleton organization and biogenesis; actin filament depolymerization; axon guidance; blood coagulation; cytokinesis after mitosis; cytoskeleton organization and biogenesis; ephrin receptor signaling pathway; establishment of cell polarity; innate immune response; negative regulation of apoptosis; neural crest cell migration; neural fold formation; platelet activation; platelet degranulation; positive regulation of actin filament depolymerization; protein amino acid phosphorylation; regulation of cell morphogenesis; response to amino acid stimulus; response to virus; Rho protein signal transduction
NCBI Summary:
The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM, Apr 2004]
