The CFL1 Polyclonal Antibody (CAB1704) is an essential research tool for studying the CFL1 protein, a key regulator of actin dynamics involved in cell migration, cytokinesis, and cell shape changes. This antibody, produced in rabbits, exhibits high specificity and reactivity with human samples, making it a reliable choice for Western blot applications.CFL1, also known as Cofilin-1, is a critical protein in cytoskeletal regulation, playing a crucial role in various cellular processes such as cell motility and cell division. Dysregulation of CFL1 has been implicated in various diseases, including cancer and neurological disorders, highlighting its importance in cellular function and disease progression.
By utilizing the CFL1 Polyclonal Antibody, researchers can investigate the expression and localization of CFL1 in different cell types and tissues, providing valuable insights into its role in normal cellular function and disease pathways. This antibody is a valuable tool for studies in cell biology, cancer research, and neurobiology, offering a deeper understanding of CFL1 and its impact on cellular processes.
Product Name:
CFL1 Rabbit Polyclonal Antibody
SKU:
CAB1704
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CFL1 (NP_005498.1).
The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.
Purification Method:
Affinity purification
Gene ID:
1072
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using CFL1 antibody (CAB1704) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.
