The Centrin-3 Polyclonal Antibody (CAB8111) is a valuable tool for researchers studying Centrin-3, a protein involved in cell division and ciliary function. This antibody, produced in rabbits, demonstrates high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the Centrin-3 protein, this antibody enables precise detection and analysis in various cell types, making it an ideal choice for investigations in cell biology and developmental biology.
Centrin-3 is known for its role in centriole duplication and cilia formation, processes essential for cell division and sensory functions. Research into Centrin-3 is important for understanding mechanisms underlying cell cycle regulation and ciliogenesis, with implications for diseases like cancer and ciliopathies. The Centrin-3 Polyclonal Antibody offers researchers a reliable tool for exploring the functions of Centrin-3 and its potential contributions to cellular processes and disease states.
Product Name:
CETN3 Rabbit Polyclonal Antibody
SKU:
CAB8111
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-167 of human CETN3 (NP_004356.2).
Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center, nucleolus.
Calculated MW:
20kDa
Observed MW:
20kDa
The protein encoded by this gene contains four EF-hand calcium binding domains, and is a member of the centrin protein family. Centrins are evolutionarily conserved proteins similar to the CDC31 protein of S. cerevisiae. Yeast CDC31 is located at the centrosome of interphase and mitotic cells, where it plays a fundamental role in centrosome duplication and separation. Multiple forms of the proteins similar to the yeast centrin have been identified in human and other mammalian cells, some of which have been shown to be associated with centrosome fractions. This protein appears to be one of the most abundant centrins associated with centrosome, which suggests a similar function to its yeast counterpart. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
1070
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using CETN3 Rabbit pAb (CAB8111) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
