The CERS3 Polyclonal Antibody (PAC019925) is a valuable tool for researchers studying CERS3, a key enzyme involved in ceramide synthesis. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the CERS3 protein, this antibody enables accurate detection and analysis in various cell types, making it an essential component for studies in lipid metabolism and lipid signaling pathways.CERS3, also known as ceramide synthase 3, plays a crucial role in maintaining cellular membrane integrity and regulating cell growth and apoptosis.
Dysregulation of ceramide synthesis has been implicated in various diseases, including cancer, metabolic disorders, and neurodegenerative diseases. Investigating the function of CERS3 using this antibody can provide valuable insights into the underlying mechanisms of these conditions and potentially lead to the development of novel therapeutic strategies targeting ceramide metabolism.
Antibody Name:
CERS3 Antibody (PACO19925)
Antibody SKU:
PACO19925
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:1000-1:2000, WB:1:200-1:1000
Species Reactivity:
Human
Immunogen:
Synthetic peptide of human CERS3
Form:
Liquid
Storage Buffer:
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:
Antigen affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane: TM4 cells, Primary antibody: PACO19925(CERS3 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 minutes.
Background:
LASS3 is a 383 amino acid, membrane protein almost exclusively expressed in testis, suggesting that LASS3 plays an important role in proper testis function. It is also weakly expressed in skin. A transcriptional variant of LASS3 cDNA exists and can result in the production of a 419 amino acid, protein (LASS3-long). LASS3 overproduction raises the level of several ceramide species, including C18:0- and C24:0-ceramides.
Synonyms:
ceramide synthase 3
UniProt Protein Function:
Has (dihydro)ceramide synthesis activity with relatively broad substrate specificity, but a preference for C18:0 and other middle- to long-chain fatty acyl-CoAs (). It is crucial for the synthesis of very long-chain ceramides in the epidermis, to maintain epidermal lipid homeostasis and terminal differentiation.
UniProt Protein Details:
NCBI Summary:
This gene is a member of the ceramide synthase family of genes. The ceramide synthase enzymes regulate sphingolipid synthesis by catalyzing the formation of ceramides from sphingoid base and acyl-coA substrates. This family member is involved in the synthesis of ceramides with ultra-long-chain acyl moieties (ULC-Cers), important to the epidermis in its role in creating a protective barrier from the environment. The protein encoded by this gene has also been implicated in modification of the lipid structures required for spermatogenesis. Mutations in this gene have been associated with male fertility defects, and epidermal defects, including ichthyosis. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2015]
