The CEP290 Polyclonal Antibody (CAB16147) is a valuable tool for researchers studying CEP290, a key protein involved in ciliogenesis and ciliopathies. The antibody, generated in rabbits, exhibits high specificity and sensitivity for detecting CEP290 in human samples, making it ideal for use in Western blot and immunohistochemistry applications.CEP290 is a centrosomal protein essential for cilia formation and function, playing a critical role in various cellular processes such as cell division and signaling. Mutations in the CEP290 gene have been linked to a range of ciliopathies, including Joubert syndrome and Leber congenital amaurosis, highlighting the importance of understanding its function.
By utilizing the CEP290 Polyclonal Antibody, researchers can further investigate the role of CEP290 in cilium assembly, intraflagellar transport, and ciliary signaling pathways. This antibody offers new insights into the molecular mechanisms underlying ciliopathies and provides a valuable tool for drug discovery efforts targeting CEP290-related disorders.
Product Name:
CEP290 Rabbit Polyclonal Antibody
SKU:
CAB16147
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 950-1050 of human CEP290 (NP_079390.3).
This gene encodes a protein with 13 putative coiled-coil domains, a region with homology to SMC chromosome segregation ATPases, six KID motifs, three tropomyosin homology domains and an ATP/GTP binding site motif A. The protein is localized to the centrosome and cilia and has sites for N-glycosylation, tyrosine sulfation, phosphorylation, N-myristoylation, and amidation. Mutations in this gene have been associated with Joubert syndrome and nephronophthisis and the presence of antibodies against this protein is associated with several forms of cancer.
Purification Method:
Affinity purification
Gene ID:
80184
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using CEP290 Rabbit pAb (CAB16147) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
