The CENPM Polyclonal Antibody (PACO16010) is a valuable tool for research involving the centromere protein M (CENPM), a key component of the centromere complex essential for proper chromosome segregation during cell division. The antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications.CENPM is crucial for maintaining genomic stability and accurate chromosome segregation, making it a critical player in cell division and cellular health. Dysregulation of CENPM has been linked to chromosomal instability and cancer development, highlighting its importance in both normal cellular function and disease states.
This antibody binds specifically to CENPM, allowing for accurate detection and analysis of this important protein in various cell types. Its high reactivity and specificity make it an ideal tool for researchers studying cell division, chromosomal dynamics, and cancer biology. By understanding the role of CENPM in these processes, researchers can gain valuable insights into the mechanisms underlying disease development and potentially identify new therapeutic targets.
Antibody Name:
CENPM Antibody (PACO16010)
Antibody SKU:
PACO16010
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:1000-1:2000, IHC:1:10-1:50
Species Reactivity:
Human
Immunogen:
Full length fusion protein
Form:
Liquid
Storage Buffer:
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:
Antigen affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using PACO16010(CENPM Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: x200).
The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using PACO16010(CENPM Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: x200).
Background:
The centromere is a specialized chromatin domain, present throughout the cell cycle, that acts as a platform on which the transient assembly of the kinetochore occurs during mitosis. All active centromeres are characterized by the presence of long arrays of nucleosomes in which CENPA (MIM 117139) replaces histone H3 (see MIM 601128). CENPM is an additional factor required for centromere assembly (Foltz et al., 2006 [PubMed 16622419]).
Synonyms:
centromere protein M
UniProt Protein Function:
CENPM: Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. 3 isoforms of the human protein are produced by alternative splicing.Chromosomal Location of Human Ortholog: 22q13.2Cellular Component: nucleoplasm; cytosolBiological Process: nucleosome assembly; DNA replication-independent nucleosome assembly at centromere; mitotic cell cycle
UniProt Protein Details:
NCBI Summary:
The centromere is a specialized chromatin domain, present throughout the cell cycle, that acts as a platform on which the transient assembly of the kinetochore occurs during mitosis. All active centromeres are characterized by the presence of long arrays of nucleosomes in which CENPA (MIM 117139) replaces histone H3 (see MIM 601128). CENPM is an additional factor required for centromere assembly (Foltz et al., 2006 [PubMed 16622419]).[supplied by OMIM, Mar 2008]
centromere protein M; interphase centromere complex protein 39; proliferation associated nuclear element 1; proliferation-associated nuclear element protein 1
UniProt Protein Name:
Centromere protein M
UniProt Synonym Protein Names:
Interphase centromere complex protein 39; Proliferation-associated nuclear element protein 1
