The CENPE Polyclonal Antibody (CAB15263) is a valuable tool for researchers studying CENPE, a protein involved in cell division and chromosome segregation. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to the CENPE protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it ideal for studies in cell biology and cancer research.
CENPE is a crucial player in mitosis, ensuring the proper alignment and segregation of chromosomes during cell division. Its role in maintaining genomic stability and proper cell division makes it a key target for research into cancer and other diseases related to cell proliferation. By understanding the function and regulation of CENPE, researchers can uncover potential therapeutic targets for cancer treatment and other disorders related to cell division.
Product Name:
CENPE Rabbit Polyclonal Antibody
SKU:
CAB15263
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 2542-2701 of human CENPE (NP_001804.2).
Centrosome-associated protein E (CENPE) is a kinesin-like motor protein that accumulates in the G2 phase of the cell cycle. Unlike other centrosome-associated proteins, it is not present during interphase and first appears at the centromere region of chromosomes during prometaphase. This protein is required for stable spindle microtubule capture at kinetochores which is a necessary step in chromosome alignment during prometaphase. This protein also couples chromosome position to microtubule depolymerizing activity. Alternative splicing results in multiple transcript variants encoding distinct protein isoforms.
Purification Method:
Affinity purification
Gene ID:
1062
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using CENPE Rabbit pAb (CAB15263) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
