The CENPA Polyclonal Antibody (CAB15995) is a valuable tool for researchers studying CENP-A, a key protein involved in chromatin organization and cell division. This antibody, generated in rabbits, exhibits high reactivity with human samples and is specifically validated for use in Western blot applications. By targeting the CENP-A protein, this antibody allows for precise detection and analysis of CENP-A in various cell types, making it an ideal choice for studies in molecular biology and cancer research.
CENP-A is a critical player in ensuring the accurate segregation of chromosomes during cell division, making it a focal point for understanding processes like mitosis and cell proliferation. Dysregulation of CENP-A has been implicated in various types of cancer, making it a promising target for drug development and therapeutic interventions. By investigating the function and regulation of CENP-A, researchers can gain valuable insights into cancer biology and potential treatment strategies.
Product Name:
CENPA Rabbit Polyclonal Antibody
SKU:
CAB15995
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CENPA (NP_001800.1).
Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. This gene encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. Centromere protein A is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle. The protein is a replication-independent histone that is a member of the histone H3 family. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Purification Method:
Affinity purification
Gene ID:
1058
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using CENPA antibody (CAB15995) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.
