Cell-Based Phosphorylation ELISA Kits

Products Overview Video Technology

Assay Genie Cell-Based Phosphorylation ELISA Kits

Forget about Western Blotting with the Assay Genie Cell-Based Phosphorylation ELISA Kits! These convenient, lysate-free 96-well ELISA can determine target protein phosphorylation levels via an indirect, in vitro immunohistochemistry based technique. This is an ideal technology to screen for the effects of kinase inhibitors & activators directly in cell culture directly without the need to make cell lysates.

Features

  • Throughput Detect protein phosphorylation status directly with 96-well ELISA technology
  • Rapid Screen in situ with no lysate preparation required
  • Convenient Assess the effects of kinase activators and inhibitors
  • Adaptable Analyse proteins in adherent and suspension cells cells

Popular Cell-Based Phosphorylation ELISA Kits

14-3-3 beta/zeta (Phospho-Ser184/186) FAK (Phospho-Tyr397) p47 phox (Phospho-Ser304)
14-3-3 theta/tau (Phospho-Ser232) FAK (Phospho-Tyr407) p47 phox (Phospho-Ser359)
14-3-3 zeta (Phospho-Ser58) FAK (Phospho-Tyr576) p53 (Phospho-Ser15)
14-3-3 zeta/delta (Phospho-Thr232) FAK (Phospho-Tyr861) p53 (Phospho-Ser20)
4E-BP1 (Phospho-Ser64) FAK (Phospho-Tyr925) p53 (Phospho-Ser315)
4E-BP1 (Phospho-Thr36) FANCD2 (Phospho-Ser222) p53 (Phospho-Ser33)
4E-BP1 (Phospho-Thr45) FER (Phospho-Tyr402) p53 (Phospho-Ser366)
4E-BP1 (Phospho-Thr69) FGFR1 (Phospho-Tyr154) p53 (Phospho-Ser37)
53BP1 (Phospho-Ser6) FGFR1 (Phospho-Tyr654) p53 (Phospho-Ser46)
ABL1 (Phospho-Thr735) FGFR1 (Phospho-Tyr766) p53 (Phospho-Ser6)
ABL1 (Phospho-Tyr204) FKHRL1 (Phospho-Ser253) p53 (Phospho-Ser9)
ABL1 (Phospho-Tyr245) FLT3 (Phospho-Tyr599) p53 (Phospho-Thr18)
ACK1 (Phospho-Tyr284) FLT3 (Phospho-Tyr842) p56 Dok-2 (Phospho-Tyr299)
ADD1/2 (Phospho-Ser726) FLT3 (Phospho-Tyr969) p57 Kip2 (Phospho-Thr310)
Adrenergic Receptor B2 (Phospho-Ser355+Ser356) FOS (Phospho-Ser32) p62 Dok (Phospho-Tyr362)
Adrenergic Receptor beta2 (Phospho-Ser346) Fos (Phospho-Ser362) p62 Dok (Phospho-Tyr398)
AhR (Phospho-Ser36) FOS (Phospho-Thr232) p63 (Phospho-Ser395)
AIRE (Phospho-Ser156) FOXO1A (Phospho-Ser256) p63 (Phospho-Ser455)
Akt1 (Phospho-Ser124) FOXO1A (Phospho-Ser319) p70 S6 Kinase (Phospho-Ser371)
Akt1 (Phospho-Ser246) FOXO1A (Phospho-Ser329) p70 S6 Kinase (Phospho-Ser411)
Akt1 (Phospho-Ser473) FOXO4 (Phospho-Ser197) p70 S6 Kinase (Phospho-Ser418)
Akt1 (Phospho-Thr308) FOXO4 (Phospho-Ser262) p70 S6 Kinase (Phospho-Ser424)
Akt1 (Phospho-Thr72) FOXO4 (Phospho-Thr451) p70 S6 Kinase (Phospho-Thr229)
Akt1 (Phospho-Tyr326) FRS2 (Phospho-Tyr436) p70 S6 Kinase beta (Phospho-Ser423)
Akt1 (Phospho-Tyr474) Fyn (Phospho-Tyr530) p73 (Phospho-Tyr99)
Akt1 S1 (Phospho-Thr246) G3BP-1 (Phospho-Ser232) p90 RSK (Phospho-Ser380)
AKT1/3 (Phospho-Tyr437/434) GAB1 (Phospho-Tyr627) p90 RSK (Phospho-Thr359+Ser363)
Akt2 (Phospho-Ser474) Gab2 (Phospho-Ser623) p90 RSK (Phospho-Thr573)
ALK (Phospho-Tyr1507) Gab2 (Phospho-Tyr643) p95-NBS1 (Phospho-Ser278)
ALK (Phospho-Tyr1604) GATA1 (Phospho-Ser142) p95-NBS1 (Phospho-Ser343)
ALOX5 (Phospho-Ser271) GATA1 (Phospho-Ser310) PAK1 (Phospho-Ser204)
AML1 (Phospho-Ser276) GATA4 (Phospho-Ser262) PAK1 (Phospho-Thr212)
AML1 (Phospho-Ser303) GIRK1/KIR3.1/KCNJ3 (Phospho-Ser185) PAK1/2/3 (Phospho-Ser144/141/139)
AML1 (Phospho-Ser435) GluR1 (Phospho-Ser863) PAK2 (Phospho-Ser141)
AMPK beta1 (Phospho-Ser181) GluR2 (Phospho-Ser880) PAK2 (Phospho-Ser192)
AMPK1 (Phospho-Ser485) GluR4 (Phospho-Ser862) PAK2 (Phospho-Ser20)
AMPKalpha (Phospho-Thr172) Glycogen Synthase (Phospho-Ser645) PAK3 (Phospho-Ser154)
Amyloid beta A4 (Phospho-Thr743/668) GR (Phospho-Ser211) Paxillin (Phospho-Tyr118)
Androgen Receptor (Phospho-Tyr363) GR (Phospho-Ser226) Paxillin (Phospho-Tyr31)
APC (Phospho-Ser2054) GRB10 (Phospho-Tyr67) PBK/TOPK (Phospho-Thr9)
A-RAF (Phospho-Tyr302) GRB2 (Phospho-Ser159) PDCD4 (Phospho-Ser457)
ARHGEF2 (Phospho-Ser885) GRK1 (Phospho-Ser21) PDCD4 (Phospho-Ser67)
Arrestin 1 (Phospho-Ser412) GRK2 (Phospho-Ser29) PDE4D (Phospho-Ser190/53)
ASK1 (Phospho-Ser83) GRK2 (Phospho-Ser685) PDGFRa (Phospho-Tyr849)
ASK1 (Phospho-Ser966) GSK3alpha (Phospho-Ser21) PDGFRalpha (Phospho-Tyr713)
ATF1 (Phospho-Ser63) GSK3alpha/beta (Phospho-Tyr279/216) PDGFRbeta (Phospho-Tyr740)
ATF2 (Phospho-Ser112) GSK3beta (Phospho-Ser9) PDK1 (Phospho-Ser241)
ATF2 (Phospho-Ser480) GTPase Activating Protein (Phospho-Ser387) PEA-15 (Phospho-Ser104)
ATF2 (Phospho-Ser62) HBP1 (Phospho-Ser402) PEA-15 (Phospho-Ser116)
ATF2 (Phospho-Thr691) HDAC1 (Phospho-Ser421) PFKFB2 (Phospho-Ser483)
ATF2 (Phospho-Thr71) HDAC2 (Phospho-Ser394) Phospho-Abl (Tyr393/412)
ATF2 (Phospho-Thr73) HDAC3 (Phospho-Ser424) Phospho-Abl (Tyr412)
ATP1A1 (Phospho-Ser16) HDAC4 (Phospho-Ser632) Phospho-BAD (Ser112)
ATP1A1 (Phospho-Ser23) HDAC5 (Phospho-Ser259) Phospho-c-Jun (Ser73)
ATP-Citrate Lyase (Phospho-Ser454) HDAC5 (Phospho-Ser498) Phospho-C-RAF (Ser642)
ATRIP (Phospho-Ser224) HDAC6 (Phospho-Ser22) Phospho-EGFR (Tyr1197)
ATRIP (Phospho-Ser68) HDAC8 (Phospho-Ser39) Phospho-EPHA2/3/4 (Tyr588/596)
AurA (Phospho-Thr288) HER2 (Phospho-Tyr1112) Phospho-GAB1 (Tyr659)
AurB (Phospho-Thr232) HER2 (Phospho-Tyr1221/Tyr1222) Phospho-HDAC7A (Ser155)
BAD (Phospho-Ser134) HER2 (Phospho-Tyr1248) Phospho-Integrin Beta1 (Tyr795)
BAD (Phospho-Ser136) HER2 (Phospho-Tyr877) Phospho-Src (Ser75)
BAD (Phospho-Ser155) HER3 (Phospho-Tyr1197) Phospho-Tau (Ser516/199)
BAD (Phospho-Ser91/128) HER3 (Phospho-Tyr1222) PI3-kinase p85-alpha/gamma (Phospho-Tyr467/199)
BCAR1 (Phospho-Tyr165) Histone H1 (Phospho-Thr17) Pim-1 (Phospho-Tyr309)
BCAR1 (Phospho-Tyr249) Histone H2A.X (Phospho-Ser139) PKA alpha/beta CAT (Phospho-Thr197)
BCAR1 (Phospho-Tyr410) Histone H3 (Phospho-Ser10) PKA-R2beta (Phospho-Ser113)
BCL2 (Phospho-Ser70) Histone H3 (Phospho-Ser28) PKC alpha (Phospho-Thr638)
BCL-2 (Phospho-Ser70) Histone H3 (Phospho-Thr3) PKC alpha (Phospho-Tyr657)
BCL-2 (Phospho-Thr56) HNF4alpha (Phospho-Ser313) PKC delta (Phospho-Ser645)
BCL-XL (Phospho-Ser62) hnRNP C1/2 (Phospho-Ser260) PKC delta (Phospho-Thr505)
BCL-XL (Phospho-Thr47) hnRNP K (Phospho-Ser284) PKC delta (Phospho-Tyr52)
Bcr (Phospho-Tyr177) hnRPD (Phospho-Ser83) PKC delta (Phospho-Tyr64)
Bcr (Phospho-Tyr360) HP1gamma (Phospho-Ser93) PKC epsilon (Phospho-Ser729)
BIK (Phospho-Thr33) HRS (Phospho-Tyr334) PKC theta (Phospho-Ser676)
BL-CAM (Phospho-Tyr807) HS1 (Phospho-Tyr397) PKC theta (Phospho-Ser695)
BLNK (Phospho-Tyr84) HSF1 (Phospho-Ser121) PKC theta (Phospho-Thr538)
BLNK (Phospho-Tyr96) HSL (Phospho-Ser552) PKC zeta (Phospho-Thr410)
B-Myb (Phospho-Ser577/581) HSL (Phospho-Ser855/554) PKC zeta (Phospho-Thr560)
B-RAF (Phospho-Ser446) HSP27 (Phospho-Ser15) PKCB (Phospho-Ser661)
B-RAF (Phospho-Ser602) HSP27 (Phospho-Ser78) PKD1/PKC mu (Phospho-Ser205)
B-Raf (Phospho-Thr753) HSP27 (Phospho-Ser82) PKD1/PKC mu (Phospho-Tyr463)
BRCA1 (Phospho-Ser1457) HSP90B (Phospho-Ser254) PKD2 (Phospho-Ser876)
Breast Tumor Kinase (Phospho-Tyr447) ICAM-1 (Phospho-Tyr512) PKR (Phospho-Thr258)
BTK (Phospho-Tyr223) ICK (Phospho-Tyr159) PKR (Phospho-Thr446)
C/EBP-alpha (Phospho-Ser21) IGF1R (Phospho-Tyr1161) PLCbeta3 (Phospho-Ser1105)
C/EBP-alpha (Phospho-Thr226) IGF1R (Phospho-Tyr1165/Tyr1166) PLCbeta3 (Phospho-Ser537)
C/EBP-beta (Phospho-Thr235/188) IkappaB-alpha (Phospho-Ser32/Ser36) PLCG1 (Phospho-Tyr1253)
C/EBP-epsilon (Phospho-Thr74) IkappaB-alpha (Phospho-Tyr305) PLCG1 (Phospho-Tyr771)
Calcium Sensing Receptor (Phospho-Thr888) IkappaB-beta (Phospho-Ser23) PLCG1 (Phospho-Tyr783)
Caldesmon (Phospho-Ser789) IkappaB-beta (Phospho-Thr19) PLCG2 (Phospho-Tyr1217)
Calnexin (Phospho-Ser583) IkappaB-epsilon (Phospho-Ser22) PLCG2 (Phospho-Tyr753)
Calsenilin/KCNIP3 (Phospho-Ser63) IKK-alpha (Phospho-Ser176) /IKK-beta (Phospho-Ser177) PLK1 (Phospho-Ser137)
CaMK1-alpha (Phospho-Thr177) IKK-alpha (Phospho-Thr23) POLR2A (Phospho-Ser1619)
CAMK2A/CAMK2D (Phospho-Thr286) IKK-alpha/beta (Phospho-Ser180/181) PP2A-alpha (Phospho-Tyr307)
CaMK2-beta/gamma/delta (Phospho-Thr287) IKK-beta (Phospho-Tyr188) PPAR-gamma (Phospho-Ser112)
CaMK4 (Phospho-Thr196/200) IKK-beta (Phospho-Tyr199) Progesterone Receptor (Phospho-Ser190)
Casein Kinase I alpha (Phospho-Tyr294) IKK-gamma (Phospho-Ser31) Progesterone Receptor (Phospho-Ser294)
Casein Kinase I alpha (Phospho-Tyr321) IKK-gamma (Phospho-Ser85) Progesterone Receptor (Phospho-Ser400)
Casein Kinase II alpha (Phospho-Tyr255) IL-10Ralpha (Phospho-Tyr496) PSEN1 (Phospho-Ser357)
Caspase 1 (Phospho-Ser376) IL-13Ralpha1 (Phospho-Tyr405) PTEN (Phospho-Ser370)
Caspase 3 (Phospho-Ser150) IL-2Rbeta (Phospho-Tyr364) PTEN (Phospho-Ser380/Thr382/Thr383)
Caspase 6 (Phospho-Ser257) IL-3Rbeta (Phospho-Tyr593) PTP1B (Phospho-Ser50)
Caspase 8 (Phospho-Ser347) IL-4R/CD124 (Phospho-Tyr497) PYK2 (Phospho-Tyr579)
Caspase 8 (Phospho-Tyr380) IL-7R/CD127 (Phospho-Tyr449) PYK2 (Phospho-Tyr580)
Caspase 9 (Phospho-Ser144) Integrin beta1 (Phospho-Thr788) PYK2 (Phospho-Tyr881)
Caspase 9 (Phospho-Ser196) Integrin beta1 (Phospho-Thr789) Rabphilin 3A (Phospho-Ser237)
Caspase 9 (Phospho-Thr125) Integrin beta3 (Phospho-Tyr773) RAD17 (Phospho-Ser645)
Caspase 9 (Phospho-Tyr153) Integrin beta3 (Phospho-Tyr785) RAD51 (Phospho-Tyr315)
Catenin-beta (Phospho-Ser37) Integrin beta4 (Phospho-Tyr1510) RAD52 (Phospho-Tyr104)
Catenin-beta (Phospho-Thr41/Ser45) Interferon-gamma Receptor alpha (Phospho-Tyr457) Rel (Phospho-Ser503)
Catenin-beta (Phospho-Tyr489) IR (Phospho-Tyr1355) RelB (Phospho-Ser573)
Catenin-beta (Phospho-Tyr654) IR (Phospho-Tyr1361) Ret (Phospho-Tyr1015)
Catenin-delta1 (Phospho-Tyr228) IRAK1 (Phospho-Thr100) Ret (Phospho-Tyr1062)
Caveolin-1 (Phospho-Tyr14) IRAK1 (Phospho-Thr209) Retinoblastoma (Phospho-Ser780)
CBL (Phospho-Tyr674) IRF-3 (Phospho-Ser385) Retinoblastoma (Phospho-Ser795)
CBL (Phospho-Tyr700) IRF-3 (Phospho-Ser386) Retinoblastoma (Phospho-Ser807)
CBL (Phospho-Tyr774) IRF-3 (Phospho-Ser396) Retinoblastoma (Phospho-Ser811)
CCR5 (Phospho-Ser336) IRS-1 (Phospho-Ser1101) Retinoblastoma (Phospho-Thr826)
CCR5 (Phospho-Ser349) IRS-1 (Phospho-Ser307) RFA2 (Phospho-Ser33)
CD19 (Phospho-Tyr531) IRS-1 (Phospho-Ser312) RFA2 (Phospho-Thr21)
CD226 (Phospho-Ser329) IRS-1 (Phospho-Ser323) RFWD2 (Phospho-Ser387)
CD3 zeta (Phospho-Tyr142) IRS-1 (Phospho-Ser612) RIPK2 (Phospho-Ser176)
CD32 (Phospho-Tyr292) IRS-1 (Phospho-Ser636) RSK1/2/3/4 (Phospho-Ser221/227/S218/232)
CD40 (Phospho-Thr254) IRS-1 (Phospho-Tyr896) S6 Ribosomal Protein (Phospho-Ser235)
CD5 (Phospho-Tyr453) JAK1 (Phospho-Tyr1022) S6 Ribosomal Protein (Phospho-Ser240)
CD50/ICAM-3 (Phospho-Ser518) JAK2 (Phospho-Tyr221) SEK1/MKK4 (Phospho-Ser80)
CD71/TfR (Phospho-Ser24) JAK2 (Phospho-Tyr570) SEK1/MKK4 (Phospho-Thr261)
CDC16/APC6 (Phospho-Ser560) JAK2 (Phospho-Tyr931) SEPARASE (Phospho-Ser801)
CDC2 (Phospho-Thr161) JAK3 (Phospho-Tyr785) SF1 (Phospho-Ser82)
CDC2 (Phospho-Tyr15) JNK1/2/3 (Phospho-Thr183) SGK (Phospho-Ser422)
CDC25A (Phospho-Ser124) JNK1/2/3 (Phospho-Thr183+Tyr185) SGK (Phospho-Ser78)
CDC25A (Phospho-Ser178) JunD (Phospho-Ser255) Shc (Phospho-Tyr349)
CDC25A (Phospho-Ser75) Keratin 18 (Phospho-Ser33) Shc (Phospho-Tyr427)
CDC25B (Phospho-Ser323) Keratin 18 (Phospho-Ser52) SHIP1 (Phospho-Tyr1021)
CDC25B (Phospho-Ser353) Keratin 8 (Phospho-Ser432) SHP-1 (Phospho-Tyr536)
CDC25C (Phospho-Thr48) Keratin 8 (Phospho-Ser73) SHP-2 (Phospho-Tyr542)
CDC37 (Phospho-Ser13) KIF11/Eg5 (Phospho-Thr926) SHP-2 (Phospho-Tyr580)
CDC6 (Phospho-Ser54) Kir6.2 (Phospho-Thr224) Smad1 (Phospho-Ser187)
CDK1/CDC2 (Phospho-Thr14) KIT (Phospho-Tyr703) Smad1 (Phospho-Ser465)
CDK2 (Phospho-Thr160) Kit (Phospho-Tyr721) Smad2 (Phospho-Ser250)
CDK5 (Phospho-Tyr15) KIT (Phospho-Tyr936) Smad2 (Phospho-Ser467)
CDK7 (Phospho-Thr170) KSR (Phospho-Ser392) Smad2/3 (Phospho-Thr8)
Chk1 (Phospho-Ser280) Ku70/XRCC6 (Phospho-Ser5) Smad3 (Phospho-Ser204)
Chk1 (Phospho-Ser286) Ku80/XRCC5 (Phospho-Thr714) Smad3 (Phospho-Ser208)
Chk1 (Phospho-Ser296) Lamin A/C (Phospho-Ser22) Smad3 (Phospho-Ser213)
Chk1 (Phospho-Ser301) Lamin A/C (Phospho-Ser392) Smad3 (Phospho-Ser425)
Chk1 (Phospho-Ser317) LAT (Phospho-Tyr171) Smad3 (Phospho-Thr179)
Chk2 (Phospho-Ser516) LAT (Phospho-Tyr191) SMC1 (Phospho-Ser957)
Chk2 (Phospho-Thr383) Lck (Phospho-Tyr192) SMC1 (Phospho-Ser966)
Chk2 (Phospho-Thr387) Lck (Phospho-Tyr393) SNAI1 (Phospho-Ser246)
Chk2 (Phospho-Thr68) Lck (Phospho-Tyr505) SOX-9 (Phospho-Ser181)
c-Jun (Phospho-Ser243) LIMK1/2 (Phospho-Thr505) SP1 (Phospho-Thr453)
c-Jun (Phospho-Ser63) LIMK1/2 (Phospho-Thr508) SP1 (Phospho-Thr739)
c-Jun (Phospho-Thr239) LKB1 (Phospho-Ser428) Src (Phospho-Tyr216)
c-Jun (Phospho-Thr91) LKB1 (Phospho-Thr189) Src (Phospho-Tyr418)
c-Jun (Phospho-Thr93) Lyn (Phospho-Tyr507) Src (Phospho-Tyr529)
c-Jun (Phospho-Tyr170) MAP2K7 (Phospho-Ser271) SREBP-1 (Phospho-Ser439)
CKII-beta (Phospho-Ser209) MAP2K7 (Phospho-Thr275) SRF (Phospho-Ser99)
Claudin 3 (Phospho-Tyr219) MAP3K7 (Phospho-Ser439) SSB (Phospho-Ser366)
Claudin 6 (Phospho-Tyr219) MAP3K7 (Phospho-Thr184) STAT1 (Phospho-Ser727)
Claudin 7 (Phospho-Tyr210) MAP3K7 (Phospho-Thr187) STAT1 (Phospho-Tyr701)
Coagulation Factor III (Phospho-Ser290) MAP3K8 (Phospho-Thr290) STAT2 (Phospho-Tyr690)
Cofilin (Phospho-Ser3) MAPKAPK2 (Phospho-Ser272) STAT3 (Phospho-Ser727)
Connexin 43 (Phospho-Ser367) MAPKAPK2 (Phospho-Thr222) STAT3 (Phospho-Tyr705)
Cortactin (Phospho-Tyr421) MAPKAPK2 (Phospho-Thr334) STAT4 (Phospho-Tyr693)
Cortactin (Phospho-Tyr466) MAPKAPK5 (Phospho-Thr182) STAT5A (Phospho-Ser780)
CPI17alpha (Phospho-Thr38) MARCKS (Phospho-Ser158) STAT5A/B (Phospho-Ser725/730)
C-RAF (Phospho-Ser296) MCM4 (Phospho-Ser54) STAT5A/B (Phospho-Ser731)
C-RAF (Phospho-Ser338) MDM2 (Phospho-Ser166) STAT5A/B (Phospho-Tyr694)
C-RAF (Phospho-Ser43) MEF2A (Phospho-Ser408) STAT6 (Phospho-Thr645)
C-RAF (Phospho-Ser621) MEF2A (Phospho-Thr312) STAT6 (Phospho-Tyr641)
C-RAF (Phospho-Tyr341) MEF2A (Phospho-Thr319) Stathmin 1 (Phospho-Ser15)
CREB (Phospho-Ser121) MEF2C (Phospho-Ser396) Stathmin 1 (Phospho-Ser24)
CREB (Phospho-Ser133) MEK1 (Phospho-Ser298) Stathmin 1 (Phospho-Ser37)
CREB (Phospho-Thr100) MEK1 (Phospho-Thr286) Survivin (Phospho-Thr34)
CrkII (Phospho-Tyr221) MEK1 (Phospho-Thr291) SYK (Phospho-Tyr323)
CrkL (Phospho-Tyr207) MEK1/2 (Phospho-Ser217) SYK (Phospho-Tyr348)
CRMP2 (Phospho-Thr509) MEK1/2 (Phospho-Ser221) SYK (Phospho-Tyr525)
CRMP2 (Phospho-Thr514) MEK2 (Phospho-Thr394) Synapsin1 (Phospho-Ser62)
CRYAB (Phospho-Ser19) Merlin (Phospho-Ser518) Synapsin1 (Phospho-Ser9)
CRYAB (Phospho-Ser45) Met (Phospho-Tyr1234) Synaptotagmin (Phospho-Ser309)
CSFR (Phospho-Tyr561) Met (Phospho-Tyr1349) Synaptotagmin (Phospho-Thr202)
CSFR (Phospho-Tyr723) Met (Phospho-Tyr1356) Synuclein-alpha (Phospho-Ser129)
Csk (Phospho-Ser364) MITF (Phospho-Ser180/73) Synuclein-alpha (Phospho-Tyr133)
CtBP1 (Phospho-Ser422) MKK3 (Phospho-Ser189) Synuclein-alpha (Phospho-Tyr136)
CXCR4 (Phospho-Ser339) MKK3 (Phospho-Thr222) Tau (Phospho-Ser214)
Cyclin B1 (Phospho-Ser126) MKK6 (Phospho-Ser207) Tau (Phospho-Ser235)
Cyclin B1 (Phospho-Ser147) MKP-1/2 (Phospho-Ser296/318) Tau (Phospho-Ser262)
Cyclin D1 (Phospho-Thr286) MNK1 (Phospho-Thr255) Tau (Phospho-Ser356)
Cyclin D3 (Phospho-Thr283) MNK1 (Phospho-Thr385) Tau (Phospho-Ser396)
Cyclin H (Phospho-Thr315) Moesin/Ezrin/Radixin (Phospho-Thr558) Tau (Phospho-Ser404)
Dab1 (Phospho-Tyr232) MRE11 (Phospho-Ser264) Tau (Phospho-Ser422)
DAPK3 (Phospho-Thr265) MSK1 (Phospho-Ser212) Tau (Phospho-Thr181)
DAPP1 (Phospho-Tyr139) MSK1 (Phospho-Ser376) Tau (Phospho-Thr205)
DARPP-32 (Phospho-Thr34) MSK1 (Phospho-Thr581) Tau (Phospho-Thr231)
DARPP-32 (Phospho-Thr75) Mst1/2 (Phospho-Thr183) Tau (Phospho-Thr534/217)
Daxx (Phospho-Ser668) mTOR (Phospho-Ser2448) TEBP (Phospho-Ser113)
Dematin (Phospho-Ser403) mTOR (Phospho-Thr2446) Telomerase (Phospho-Ser824)
Dynamin-1 (Phospho-Ser774) MYB (Phospho-Ser12) TERF1 (Phospho-Ser219)
Dynamin-1 (Phospho-Ser778) Myc (Phospho-Ser62) TIE2 (Phospho-Tyr1108)
E2F1 (Phospho-Thr433) Myc (Phospho-Thr58) TIF-IA (Phospho-Ser649)
eEF2 (Phospho-Thr56) Myogenic Factor 5 (Phospho-Ser49) TIP60 (Phospho-Ser86)
eEF2K (Phospho-Ser366) Myosin regulatory light chain 2 (Phospho-Ser18) TISB (Phospho-Ser92)
EFNB1 (Phospho-Tyr317) MYPT1 (Phospho-Thr853) TK (Phospho-Ser13)
EGFR (Phospho-Ser1026) NEK9 (Phospho-Thr210) TNNI3 (Phospho-Ser43)
EGFR (Phospho-Ser1070) NFAT3 (Phospho-Ser676) TNNI3 (Phospho-Thr142)
EGFR (Phospho-Ser1071) NFAT4 (Phospho-Ser165) TOB1 (Phospho-Ser164)
EGFR (Phospho-Ser695) NF-kappaB p100/p52 (Phospho-Ser865) TOP2A (Phospho-Ser1106)
EGFR (Phospho-Thr678) NF-kappaB p100/p52 (Phospho-Ser869) TOP2A (Phospho-Ser1525)
EGFR (Phospho-Thr693) NF-kappaB p100/p52 (Phospho-Ser872) TOP2A (Phospho-Thr1343)
EGFR (Phospho-Tyr1016) NF-kappaB p105/p50 (Phospho-Ser337) Trk A (Phospho-Tyr680+Tyr681)
EGFR (Phospho-Tyr1069) NF-kappaB p105/p50 (Phospho-Ser893) Trk A (Phospho-Tyr701)
EGFR (Phospho-Tyr1092) NF-kappaB p105/p50 (Phospho-Ser907) Trk B (Phospho-Tyr515)
EGFR (Phospho-Tyr1110) NF-kappaB p105/p50 (Phospho-Ser927) Trk B (Phospho-Tyr705)
EGFR (Phospho-Tyr1172) NF-kappaB p105/p50 (Phospho-Ser932) Tryptophan Hydroxylase (Phospho-Ser260)
EGFR (Phospho-Tyr869) NF-kappaB p65 (Phospho-Ser276) Tryptophan Hydroxylase (Phospho-Ser58)
eIF2alpha (Phospho-Ser51) NF-kappaB p65 (Phospho-Ser311) Tuberin/TSC2 (Phospho-Ser939)
eIF4B (Phospho-Ser422) NF-kappaB p65 (Phospho-Ser468) Tyrosine Hydroxylase (Phospho-Ser19)
Elk1 (Phospho-Ser383) NF-kappaB p65 (Phospho-Ser529) Tyrosine Hydroxylase (Phospho-Ser31)
Elk1 (Phospho-Ser389) NF-kappaB p65 (Phospho-Ser536) Tyrosine Hydroxylase (Phospho-Ser40)
eNOS (Phospho-Ser1176) NF-kappaB p65 (Phospho-Thr254) Tyrosine Hydroxylase (Phospho-Ser8)
eNOS (Phospho-Ser615) NF-kappaB p65 (Phospho-Thr435) VASP (Phospho-Ser157)
eNOS (Phospho-Thr494) NIPA (Phospho-Ser354) VASP (Phospho-Ser238)
EPB41 (Phospho-Tyr660/418) NMDAR1 (Phospho-Ser896) VAV1 (Phospho-Tyr174)
EPHB1/2 (Phospho-Tyr594/604) NMDAR1 (Phospho-Ser897) VAV2 (Phospho-Tyr142)
Ephrin B1/B2 (Phospho-Tyr330) NMDAR2B (Phospho-Ser1303) VCP (Phospho-Ser352)
Epo-R (Phospho-Tyr368) NMDAR2B (Phospho-Tyr1336) VEGFR2 (Phospho-Tyr1059)
ERK3 (Phospho-Ser189) NMDAR2B (Phospho-Tyr1474) VEGFR2 (Phospho-Tyr1175)
Estrogen Receptor-alpha (Phospho-Ser104) nNOS (Phospho-Ser852) VEGFR2 (Phospho-Tyr1214)
Estrogen Receptor-alpha (Phospho-Ser106) NPM (Phospho-Thr199) VEGFR2 (Phospho-Tyr951)
Estrogen Receptor-alpha (Phospho-Ser118) NPM (Phospho-Thr234) Vimentin (Phospho-Ser56)
Estrogen Receptor-alpha (Phospho-Ser167) NudC (Phospho-Ser326) Vimentin (Phospho-Ser83)
Estrogen Receptor-alpha (Phospho-Tyr537) Opioid Receptor-delta (Phospho-Ser363) Vitamin D Receptor (Phospho-Ser208)
Estrogen Receptor-beta (Phospho-Ser105) p21 Cip1 (Phospho-Thr145) WASP (Phospho-Tyr290)
ETK (Phospho-Tyr40) p27 Kip1 (Phospho-Ser10) WAVE1 (Phospho-Tyr125)
ETK (Phospho-Tyr566) p27 Kip1 (Phospho-Thr187) WEE1 (Phospho-Ser642)
ETS1 (Phospho-Thr38) p27 Kip1 (Phospho-Thr198) WNK1 (Phospho-Thr58)
Ezrin (Phospho-Tyr353) p38 MAPK (Phospho-Thr180) WWOX (Phospho-Tyr33)
Ezrin (Phospho-Tyr478) p38 MAPK (Phospho-Tyr182) YAP (Phospho-Ser127)
FADD (Phospho-Ser191) p38 MAPK (Phospho-Tyr322) ZAP-70 (Phospho-Tyr292)
FADD (Phospho-Ser194) p44/42 MAP Kinase (Phospho-Thr202) ZAP-70 (Phospho-Tyr315)
FAK (Phospho-Ser843) p44/42 MAP Kinase (Phospho-Tyr204) ZAP-70 (Phospho-Tyr493)

Cell-Based Phosphorylation ELISA Overview

Key Cell-Based Phospho ELISA Kits


Contents

Fluorometric Cell-Based Phospho ELISA Kit

  • TBS
  • Quenching Buffer
  • Blocking Buffer
  • Wash Buffer
  • Primary Antibody Diluent
  • α-Phospho-Target Antibody
  • α-Target Antibody
  • α-GAPDH antibody
  • Dye-1 Conjugated Anti-Rabbit IgG Antibody
  • Dye-2 Conjugated Anti-Mouse IgG Antibody
  • 96-Well Black Cell Culture Clear-Bottom Microplate
  • Adhesive Plate Seals

Colorimetric Cell-Based Phospho ELISA Kit

  • TBS
  • Quenching Buffer
  • Blocking Buffer
  • Wash Buffer
  • Primary Antibody Diluent
  • α-Phospho-Target Antibody
  • α-Target Antibody
  • HRP-Conjugated Anti-Rabbit IgG Antibody
  • HRP-Conjugated Anti-Mouse IgG Antibody
  • Crystal Violet Solution
  • SDS Solution
  • Ready-to-Use Substrate
  • Stop Solution
  • 96-Well Cell Culture Clear-Bottom Microplate
  • Adhesive Plate Seals

Applications

  • Quantification of phosphorylated proteins in whole cell cultures

  • Investigation of cell signaling pathways

  • Investigation of pathway activation of other cellular processes (e.g. cell cycle)

Cell-Based Phosphorylation ELISA Principle

Fluorometric Phospho ELISA

For Fluorometric Cell-Based Phospho ELISA, the target phosphorylated protein is captured by phospho-specific primary (1°) antibodies while Dye 1-conjugated and Dye 2-conjugated secondary (2°) antibodies bind the Fc region of the 1° antibody. Through this binding, the dye conjugated to the 2° antibody can emit light at a certain wavelength given proper excitation, hence allowing for a fluorometric detection method.

A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target RFU values. If a phosphorylated target is being detected, an antibody against the nonphosphorylated counterpart will be provided for normalization purposes.

Figure : Assay format of Assay Genie Fluorometric Cell-Based Phosphorylation ELISA Kit

Figure : Assay format of Assay Genie Colorimetric Cell-Based Phosphorylation ELISA Kit

Colorimetric Phospho ELISA

For Colorimetric Cell-Based Phospho ELISA, the target phosphorylated protein is captured by phospho-specific primary (1°) antibodies while the HRP-conjugated secondary (2°) antibodies bind the Fc region of the 1° antibody. Through this binding, the HRP enzyme conjugated to the 2° antibody can catalyze a colorimetric reaction upon substrate addition.