Cell-Based ELISA Kits
Cell-Based ELISA Kits
No More Western Blots!
Over 1,600 Cell-Based ELISA kits to measure the relative levels of intracellular and phosphorylated proteins!
Cell-Based ELISA Features
- Throughput | High throughput screening using 96-well ELISA technology
- Rapid | Screen in situ with no lysate preparation required
- Convenient | Assess the effects of activators and inhibitors on cellular processes
- Adaptable | Analyse proteins in adherent and suspension cells
- Phosphorylation | Range of Cell-Based Phospho kits for quantification of phosphorylated proteins in whole cell cultures!
Cell-Based ELISA Kits
Colorimetric Cell-Based ELISA
Colorimetric Cell-Based Phosphorylation ELISA
Fluorometric Cell-Based Phosphorylation ELISA
What's the difference? Colorimetric vs. Fluorometric
Schematic of Colorimetric ELISA Principle
Colorimetric Cell-Based ELISA
For Colorimetric ELISA, the target protein is captured by target-specific primary antibodies while the HRP-conjugated secondary antibodies bind the Fc region of the primary antibody. Through this binding, the HRP enzyme conjugated to the secondary antibody can catalyze a colorimetric reaction upon substrate addition.
Fluorometric Cell-Based ELISA
For Fluorometric ELISA, the target protein is captured by target-specific primary antibodies while Dye 1-conjugated and Dye 2-conjugated secondary antibodies bind the Fc region of the primary antibody. Through this binding, the dye conjugated to the secondary antibody can emit light at a certain wavelength given proper excitation, hence allowing for a fluorometric detection method.
A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target RFU values. If a phosphorylated target is being detected, an antibody against the nonphosphorylated counterpart will be provided for normalization purposes.
Schematic of Fluorometric ELISA Principle
Free Consultation
To see how Cell-Based ELISA Kits could support your research get in touch for a free no obligation consultation! Simply use the promo code FREECB in your message when submitting a contact form!
Cell-Based ELISA Kit Contents
Colorimetric Cell-Based ELISA
| TBS | |
| Quenching Buffer | |
| Blocking Buffer | |
| Wash Buffer | |
| Primary Antibody Diluent | |
| α-Target Antibody | |
| α-GAPDH Antibody | |
| HRP-Conjugated Anti-Rabbit IgG Antibody | |
| HRP-Conjugated Anti-Mouse IgG Antibody | |
| SDS Solution | |
| Crystal Violet Solution | |
| Ready-to-use Substrate | |
| Stop Solution | |
| 96-Well Cell Culture Clear-Bottom Microplate | |
| Adhesive Plate Seals |
Colorimetric Cell-Based Phospho ELISA
| TBS | |
| Quenching Buffer | |
| Blocking Buffer | |
| Wash Buffer | |
| Primary Antibody Diluent | |
| α-Target Antibody | |
| α-Phospho-Target Antibody | |
| HRP-Conjugated Anti-Rabbit IgG Antibody | |
| HRP-Conjugated Anti-Mouse IgG Antibody | |
| SDS Solution | |
| Crystal Violet Solution | |
| Ready-to-use Substrate | |
| Stop Solution | |
| 96-Well Cell Culture Clear-Bottom Microplate | |
| Adhesive Plate Seals |
Fluorometric Cell-Based Phospho ELISA
| TBS | |
| Quenching Buffer | |
| Blocking Buffer | |
| Wash Buffer | |
| Primary Antibody Diluent | |
| α-Phospho-Target Antibody | |
| α-Target Antibody | |
| α-GAPDH Antibody | |
| Dye-1 Conjugated Anti-Rabbit IgG Antibody | |
| Dye-2 Conjugated Anti-Mouse IgG Antibody | |
| 96-Well Black Cell Culture Clear-Bottom Microplate | |
| Adhesive Plate Seals |
Cell-Based FAQs
At Assay Genie we have compiled a list of some commonly asked questions regarding Cell-Based ELISA Kits along with their solutions!
FAQs
1.) My kit was left out at room temperature for several days. Can it still be used?
The kit may have diminished activity after an extended period at room temperature, but it is most likely still usable, especially if left out for only a small amount of time. The most temperature-sensitive components in the kit are the standards, HRP-streptavidin, and detection antibody.
2.) Why is there a weak signal/no signal detected from my Cell-Based ELISA?
This may due to a number of reasons. The possible causes and their solutions are listed below:
| Possible Cause | Solution |
|
Storage and Expiration |
Read Manual for storage condition and expiration. |
|
Antibody Dilution |
Dilute antibody 1:100 with Antibody Diluent. |
|
Low Cell Number |
Seed more cells |
|
Cells Detach from Well-Bottoms |
Add solutions slowly from the side wall of the wells. Use Poly-Lysine to coat the plate period seeding the cells, if necessary. |
3.) Why is their high background noise from my Cell-Based ELISA?
This may due to a number of reasons. The possible causes and their solutions are listed below:
| Possible Cause | Solution |
|
Washing |
Remove Wash Buffer completely |
|
High Cell Number |
Reduce the amount of seeded cells. |
4.) Why is there variation in my sample duplicate results when performing a Cell-Based ELISA?
This may due to a number of reasons. The possible causes and their solutions are listed below:
| Possible Cause | Solution |
|
Pipetting |
Check and/or calibrate pipettes. |
|
Washing |
Remove Wash Buffer completely. |
|
Cells Detach from Well-Bottoms |
Add solutions slowly from the side wall of the wells. Use Poly-L-Lysine to coat the plate period seeding the cells, if necessary. |