The CELF4 Antibody (PAC061221) is a critical tool for researchers studying CELF4, a RNA-binding protein involved in post-transcriptional regulation of gene expression. This polyclonal antibody is produced in rabbits and specifically targets CELF4 in human samples. Validated for use in Western blot applications, this antibody allows for the detection and analysis of CELF4 in various cell types, making it an ideal choice for studies in molecular biology and gene regulation.CELF4 is known to play a role in several cellular processes, including alternative splicing and mRNA stability, making it a key player in gene expression control.
Its dysregulation has been linked to various diseases, such as cancer and neurological disorders, highlighting the importance of studying its function. By using the CELF4 Antibody, researchers can gain insights into the mechanisms underlying these conditions and potentially identify novel therapeutic targets for intervention.
Antibody Name:
CELF4 Antibody (PACO61221)
Antibody SKU:
PACO61221
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:10000, IHC:1:200-1:500
Species Reactivity:
Human
Immunogen:
Recombinant Human CUGBP Elav-like family member 4 protein (120-236AA)
IHC image of PACO61221 diluted at 1:200 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
RNA-binding protein implicated in the regulation of pre-mRNA alternative splicing. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of cardiac isoforms of TNNT2 during heart remodeling at the juvenile to adult transition. Promotes exclusion of both the smooth muscle (SM) and non-muscle (NM) exons in actinin pre-mRNAs. Activates the splicing of MAPT/Tau exon 10. Binds to muscle-specific splicing enhancer (MSE) intronic sites flanking the alternative exon 5 of TNNT2 pre-mRNA.
Synonyms:
CUGBP Elav-like family member 4 (CELF-4) (Bruno-like protein 4) (CUG-BP- and ETR-3-like factor 4) (RNA-binding protein BRUNOL-4), CELF4, BRUNOL4
UniProt Protein Function:
CELF4: RNA-binding protein implicated in the regulation of pre- mRNA alternative splicing. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of cardiac isoforms of TNNT2 during heart remodeling at the juvenile to adult transition. Promotes exclusion of both the smooth muscle (SM) and non-muscle (NM) exons in actinin pre-mRNAs. Activates the splicing of MAPT/Tau exon 10. Binds to muscle-specific splicing enhancer (MSE) intronic sites flanking the alternative exon 5 of TNNT2 pre-mRNA. Belongs to the CELF/BRUNOL family. 5 isoforms of the human protein are produced by alternative splicing.Protein type: RNA splicingChromosomal Location of Human Ortholog: 18q12Cellular Component: cytoplasm; nucleusMolecular Function: mRNA binding; BRE binding; translation repressor activity, nucleic acid binding; nucleotide bindingBiological Process: positive regulation of nuclear mRNA splicing, via spliceosome; alternative nuclear mRNA splicing, via spliceosome; mRNA splice site selection; embryonic development; negative regulation of nuclear mRNA splicing, via spliceosome; negative regulation of translation; regulation of alternative nuclear mRNA splicing, via spliceosome; germ cell development
UniProt Protein Details:
NCBI Summary:
Members of the CELF/BRUNOL protein family contain two N-terminal RNA recognition motif (RRM) domains, one C-terminal RRM domain, and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing, and translation. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
