The CDKN2A Polyclonal Antibody (CAB11337) is an essential tool for researchers investigating the CDKN2A gene, which encodes for the p16 protein involved in cell cycle regulation and tumor suppression. This antibody, produced in rabbits, exhibits high specificity and reactivity towards human samples, making it ideal for Western blot applications.CDKN2A, also known as p16INK4a, functions as a tumor suppressor by inhibiting the activity of cyclin-dependent kinases and preventing aberrant cell proliferation. Dysregulation of CDKN2A has been implicated in various cancers, particularly melanoma and pancreatic cancer, highlighting its importance in cancer biology research.
By utilizing the CDKN2A Polyclonal Antibody, researchers can accurately detect and analyze the expression levels of the p16 protein in different cell types and tissue samples. This antibody's reliability and sensitivity make it a valuable tool for studies aimed at understanding the role of CDKN2A in cancer development and progression. Additionally, it can aid in the identification of potential therapeutic targets and biomarkers for cancer diagnostics and treatment strategies.
Product Name:
CDKN2A/p16INK4a Rabbit Polyclonal Antibody
SKU:
CAB11337
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-156 of human CDKN2A/p16INK4a (NP_000068.1).
This gene generates several transcript variants which differ in their first exons. At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene; this transcript contains an alternate open reading frame (ARF) that specifies a protein which is structurally unrelated to the products of the other variants. This ARF product functions as a stabilizer of the tumor suppressor protein p53 as it can interact with, and sequester, the E3 ubiquitin-protein ligase MDM2, a protein responsible for the degradation of p53. In spite of the structural and functional differences, the CDK inhibitor isoforms and the ARF product encoded by this gene, through the regulatory roles of CDK4 and p53 in cell cycle G1 progression, share a common functionality in cell cycle G1 control. This gene is frequently mutated or deleted in a wide variety of tumors, and is known to be an important tumor suppressor gene.
Purification Method:
Affinity purification
Gene ID:
1029
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using CDKN2A/p16INK4a antibody (CAB11337) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
