The CDKN2A Polyclonal Antibody (CAB0262) is a valuable tool for researchers studying the CDKN2A protein, also known as p16, which plays a pivotal role in cell cycle regulation and tumor suppression. This polyclonal antibody, raised in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications.CDKN2A is a tumor suppressor gene that encodes for the p16 protein, a key player in controlling cell cycle progression and preventing abnormal cell proliferation. Dysregulation of CDKN2A/p16 is commonly observed in various cancers, making it an important target for cancer research.
The CDKN2A Polyclonal Antibody enables precise detection and analysis of CDKN2A protein levels in different cell types, providing valuable insights into its role in cancer development and progression.Researchers studying oncology, molecular biology, and cell cycle regulation will benefit from using the CDKN2A Polyclonal Antibody to investigate the function and expression of CDKN2A/p16 in physiological and pathological conditions. Its specificity and reliability make it an essential reagent for experiments aiming to elucidate the mechanisms underlying cancer formation and progression.
This gene generates several transcript variants which differ in their first exons. At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene; this transcript contains an alternate open reading frame (ARF) that specifies a protein which is structurally unrelated to the products of the other variants. This ARF product functions as a stabilizer of the tumor suppressor protein p53 as it can interact with, and sequester, the E3 ubiquitin-protein ligase MDM2, a protein responsible for the degradation of p53. In spite of the structural and functional differences, the CDK inhibitor isoforms and the ARF product encoded by this gene, through the regulatory roles of CDK4 and p53 in cell cycle G1 progression, share a common functionality in cell cycle G1 control. This gene is frequently mutated or deleted in a wide variety of tumors, and is known to be an important tumor suppressor gene.
Purification Method:
Affinity purification
Gene ID:
1029
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using [KO Validated] CDKN2A/p16INK4a Rabbit pAb (CAB0262) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
