The CDKN2A (p16INK4a) Polyclonal Antibody (CAB11920) is a versatile tool for studying the CDKN2A gene, a key tumor suppressor gene often implicated in various cancers. This antibody, generated in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to the p16INK4a protein, researchers can accurately detect and analyze its expression levels in different cell types, making it an invaluable tool for studies in oncology and cancer biology.The CDKN2A gene, also known as cyclin-dependent kinase inhibitor 2A, encodes the p16INK4a protein which plays a crucial role in cell cycle regulation and tumor suppression. Mutations or dysregulation of CDKN2A/p16INK4a are frequently observed in cancer cells, highlighting its importance in preventing uncontrolled cell growth and malignancy.
By studying the expression and function of p16INK4a, researchers can gain insights into the mechanisms of tumorigenesis and potentially identify therapeutic targets for cancer treatment.The CDKN2A (p16INK4a) Polyclonal Antibody (CAB11920) is a valuable tool for investigating the role of CDKN2A in cancer development and progression. Its high specificity and sensitivity make it an ideal choice for researchers looking to explore the molecular mechanisms underlying tumor formation and potentially identify novel biomarkers for cancer diagnosis and prognosis. By using this
Product Name:
CDKN2A/p16INK4a Polyclonal Antibody
SKU:
CAB11920
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 50-156 of human CDKN2A/p16INK4a (NP_000068.1).
This gene generates several transcript variants which differ in their first exons. At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene; this transcript contains an alternate open reading frame (ARF) that specifies a protein which is structurally unrelated to the products of the other variants. This ARF product functions as a stabilizer of the tumor suppressor protein p53 as it can interact with, and sequester, the E3 ubiquitin-protein ligase MDM2, a protein responsible for the degradation of p53. In spite of the structural and functional differences, the CDK inhibitor isoforms and the ARF product encoded by this gene, through the regulatory roles of CDK4 and p53 in cell cycle G1 progression, share a common functionality in cell cycle G1 control. This gene is frequently mutated or deleted in a wide variety of tumors, and is known to be an important tumor suppressor gene.
Purification Method:
Affinity purification
Gene ID:
1029
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from 293 cells, using CDKN2A/p16INK4a Rabbit pAb (CAB11920).Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.
