The CDKN1B Polyclonal Antibody (CAB16633) is a valuable tool for research involving CDKN1B, a protein that plays a key role in cell cycle regulation. This antibody, raised in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. It binds to the CDKN1B protein, facilitating the detection and analysis of this important cell cycle regulator in various cell types.CDKN1B, also known as p27 or Kip1, acts as a tumor suppressor by inhibiting the activity of cyclin-dependent kinases and regulating cell cycle progression. Dysregulation of CDKN1B has been implicated in various types of cancer, making it a promising target for cancer research.
Additionally, CDKN1B is involved in diverse cellular processes, including cell differentiation and apoptosis, highlighting its significance in basic biological research.By using the CDKN1B Polyclonal Antibody, researchers can gain valuable insights into the function and regulation of CDKN1B in normal and disease states. This antibody is a valuable tool for studies in oncology, cell biology, and molecular biology, providing a means to investigate the role of CDKN1B in various physiological and pathological processes.
This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Mutations in this gene are associated with multiple endocrine neoplasia type IV (MEN4).
Purification Method:
Affinity purification
Gene ID:
1027
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using CDKN1B/p27KIP1 antibody (CAB16633) at 1:900 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 20s.
