The CDKN1B Polyclonal Antibody (CAB0290) is a valuable tool for researchers studying CDKN1B, a key regulator of cell cycle progression and proliferation. This antibody, generated in rabbits, demonstrates high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the CDKN1B protein, this antibody allows for precise detection and analysis in a variety of cell types, making it ideal for research in oncology and cell biology.CDKN1B, also known as p27Kip1, acts as a tumor suppressor by inhibiting cyclin-dependent kinases and controlling progression through the cell cycle.
Dysregulation of CDKN1B is commonly observed in cancer, making it a promising target for therapeutic interventions. Research into its mechanisms of action can provide valuable insights into the development and progression of various cancers, as well as potential strategies for targeted treatment approaches. Understanding the role of CDKN1B is crucial for advancing our knowledge of cell cycle regulation and its implications for disease.
This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Mutations in this gene are associated with multiple endocrine neoplasia type IV (MEN4).
Purification Method:
Affinity purification
Gene ID:
1027
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using CDKN1B/p27KIP1 Rabbit pAb (CAB0290) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3min.
