The CDKN1A Polyclonal Antibody (CAB2691) is a valuable tool for researchers studying the CDKN1A protein, also known as p21, a key regulator of cell cycle progression and cellular response to DNA damage. This antibody, produced using rabbit immunization, has high specificity for human samples and has been validated for use in Western blot assays. By targeting the CDKN1A protein, this antibody allows for precise detection and analysis of protein expression levels in a variety of cell types, making it ideal for investigations in cell biology and cancer research.
CDKN1A is a critical player in maintaining genomic integrity and preventing uncontrolled cell proliferation, making it a crucial factor in understanding tumorigenesis and cancer progression. Its role in cell cycle arrest and apoptosis makes it a potential target for therapeutic interventions in various cancer types. By studying the expression and activity of CDKN1A, researchers can gain insights into the mechanisms of cell growth regulation and develop novel strategies for cancer treatment.
This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or -cyclin-dependent kinase4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen, a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of cyclin-dependent kinase2, and may be instrumental in the execution of apoptosis following caspase activation. Mice that lack this gene have the ability to regenerate damaged or missing tissue. Multiple alternatively spliced variants have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
1026
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using CDKN1A/p21CIP1 Rabbit pAb (CAB2691) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
