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[KO Validated] CDKN1A/p21CIP1 Rabbit Polyclonal Antibody (CAB2691)

SKU:
CAB2691
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Cycle
  • KO Validated Antibodies 1 Anti-CDKN1A/p21CIP1 Antibody CAB2691KO Validated
  • KO Validated Antibodies 1 Anti-CDKN1A/p21CIP1 Antibody CAB2691KO Validated
  • KO Validated Antibodies 1 Anti-CDKN1A/p21CIP1 Antibody CAB2691KO Validated
  • KO Validated Antibodies 1 Anti-CDKN1A/p21CIP1 Antibody CAB2691KO Validated
€139 - €419
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:[KO Validated] CDKN1A/p21CIP1 Rabbit Polyclonal Antibody
SKU:CAB2691
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-164 of human CDKN1A/p21CIP1 (NP_000380.1).
Sequence:MSEP AGDV RQNP CGSK ACRR LFGP VDSE QLSR DCDA LMAG CIQE ARER WNFD FVTE TPLE GDFA WERV RGLG LPKL YLPT GPRR GRDE LGGG RRPG TSPA LLQG TAEE DHVD LSLS CTLV PRSG EQAE GSPG GPGD SQGR KRRQ TSMT DFYH SKRR LIFS KRKP
Tested Applications:WB IHC-P IP ELISA
Recommended Dilution:WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 IP,1:100 - 1:500
Synonyms:P21; CIP1; SDI1; WAF1; CAP20; CDKN1; MDA-6; p21CIP1; CDKN1A/p21CIP1
Positive Sample:MCF7,293T,Mouse spleen
Conjugate:Unconjugated
Cellular Localization:Cytoplasm, Nucleus.
Calculated MW:18kDa
Observed MW:18kDa(Mouse)/21kDa(Human/Rat)

This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or -cyclin-dependent kinase4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen, a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of cyclin-dependent kinase2, and may be instrumental in the execution of apoptosis following caspase activation. Mice that lack this gene have the ability to regenerate damaged or missing tissue. Multiple alternatively spliced variants have been found for this gene.

Purification Method:Affinity purification
Gene ID:1026
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using CDKN1A/p21CIP1 Rabbit pAb (CAB2691) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.

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