The CDKN1A Polyclonal Antibody (CAB1483) is a powerful tool for researchers studying the CDKN1A protein, which is involved in cell cycle regulation and tumor suppression. This antibody, generated in rabbits, exhibits high specificity and sensitivity towards human samples, making it an ideal choice for experiments such as Western blotting.CDKN1A, also known as p21, is a crucial player in cell cycle control, functioning as a potent inhibitor of cyclin-dependent kinases. Dysregulation of this protein has been linked to various types of cancer, making it a promising target for cancer research.
By targeting CDKN1A with this antibody, researchers can gain valuable insights into the mechanisms underlying cell cycle progression and tumor development.Overall, the CDKN1A Polyclonal Antibody is a valuable tool for researchers seeking to unravel the complexities of cell cycle regulation and cancer biology. Its high reactivity and specificity make it a reliable choice for experiments aimed at understanding the role of CDKN1A in various disease contexts.
Product Name:
CDKN1A/p21CIP1 Rabbit Polyclonal Antibody
SKU:
CAB1483
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-164 of human CDKN1A/p21CIP1 (NP_000380.1).
This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or -cyclin-dependent kinase4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen, a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of cyclin-dependent kinase2, and may be instrumental in the execution of apoptosis following caspase activation. Mice that lack this gene have the ability to regenerate damaged or missing tissue. Multiple alternatively spliced variants have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
1026
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from MCF7 cells, using CDKN1A/p21CIP1 Rabbit pAb (CAB1483) at 1:900 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
