The CDK9 Rabbit Monoclonal Antibody is a highly specific antibody designed for research involving CDK9, a key regulator of gene transcription and cell cycle progression. This antibody, produced in rabbits, is validated for use in various applications, including Western blot, immunohistochemistry, and immunofluorescence.CDK9 is a crucial component of the positive transcription elongation factor b (P-TEFb) complex, which plays a critical role in the regulation of RNA polymerase II-mediated transcription. This complex is involved in the expression of many genes, including those involved in cell growth, differentiation, and apoptosis.
The CDK9 Rabbit Monoclonal Antibody binds specifically to CDK9, enabling researchers to study its expression and localization in different cell types and tissues. Understanding the function of CDK9 is essential for elucidating its role in various biological processes, including cancer development, HIV replication, and cardiac hypertrophy.Overall, the CDK9 Rabbit Monoclonal Antibody is a valuable tool for researchers interested in exploring the mechanisms underlying gene transcription and cell cycle regulation, with potential implications for cancer biology, viral infections, and other diseases.
Product Name:
CDK9 Rabbit Monoclonal Antibody
SKU:
CAB11145
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 273-372 of human CDK9 (P50750).
WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
TAK; C-2k; CTK1; CDC2L4; PITALRE; K9
Positive Sample:
HeLa, 293T ,MCF7, Mouse brain, Rat testis
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Nucleus, PML body.
Calculated MW:
43kDa
Observed MW:
42kDa/55kDa
The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. This kinase was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS.
Purification Method:
Affinity purification
Gene ID:
1025
Clone Number:
ARC0527
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using CDK9 Rabbit mAb (CAB11145) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3min.
