The CDK9 Polyclonal Antibody (CAB0886) is a valuable tool for research related to CDK9, a cyclin-dependent kinase known for its role in regulating gene transcription and cell cycle progression. This antibody, raised in rabbits, exhibits high reactivity towards human samples and has been validated for use in Western blot applications. By binding specifically to CDK9 protein, this antibody enables accurate detection and analysis in various cell types, making it ideal for studies in molecular biology and cancer research.
CDK9 is a key player in the regulation of RNA polymerase II transcription, making it a promising target for therapeutic intervention in diseases such as cancer and HIV. By understanding the mechanisms by which CDK9 regulates gene expression, researchers can develop novel treatments that target this pathway. The CDK9 Polyclonal Antibody is a valuable tool in unraveling the complex functions of CDK9 and advancing our understanding of its role in health and disease.
Product Name:
CDK9 Rabbit Polyclonal Antibody
SKU:
CAB0886
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 153-372 of human CDK9 (NP_001252.1).
The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. This kinase was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS.
Purification Method:
Affinity purification
Gene ID:
1025
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using CDK9 Rabbit pAb (CAB0886) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
