Anti-CDK5 Antibody (CAB5730)
- SKU:
- CAB5730
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Death
Description
Anti-CDK5 Antibody (CAB5730)
The CDK5 Polyclonal Antibody (CAB5730) is a valuable tool for researchers studying the CDK5 protein, a key regulator of cell cycle progression and neuronal development. This antibody, produced in rabbits, demonstrates high reactivity with human samples and is validated for use in Western blot applications. By specifically binding to CDK5, this antibody enables the detection and analysis of the protein in various cell types, making it a versatile option for studies in cell biology, neurobiology, and cancer research.CDK5, a cyclin-dependent kinase, plays a crucial role in cell division and differentiation, as well as in neuronal development and synaptic plasticity.
Dysregulation of CDK5 has been implicated in various diseases, including cancer and neurodegenerative disorders. By understanding the function and regulation of CDK5, researchers can gain insights into disease mechanisms and potentially identify new therapeutic targets for these conditions. The CDK5 Polyclonal Antibody is a valuable tool for advancing our understanding of CDK5 biology and its implications in health and disease.
| Antibody Name: | Anti-CDK5 Antibody |
| Antibody SKU: | CAB5730 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF IP |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-292 of human CDK5 (NP_004926.1). |
| Application: | WB IF IP |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:10 - 1:100 IP 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 293T, HepG2, BT-474, A-549, PC-12, Mouse brain, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-292 of human CDK5 (NP_004926.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MQKY EKLE KIGE GTYG TVFK AKNR ETHE IVAL KRVR LDDD DEGV PSSA LREI CLLK ELKH KNIV RLHD VLHS DKKL TLVF EFCD QDLK KYFD SCNG DLDP EIVK SFLF QLLK GLGF CHSR NVLH RDLK PQNL LINR NGEL KLAD FGLA RAFG IPVR CYSA EVVT LWYR PPDV LFGA KLYS TSID MWSA GCIF AELA NAGR PLFP GNDV DDQL KRIF RLLG TPTE EQWP SMTK LPDY KPYP MYPA TTSL VNVV PKLN ATGR DLLQ NLLK CNPV QRIS AEEA LQHP YFSD FCPP |
| Gene ID: | 1020 |
| Uniprot: | Q00535 |
| Cellular Location: | Cell junction, Cell membrane, Cell projection, Cytoplasm, Nucleus, Perikaryon, Peripheral membrane protein, growth cone, lamellipodium, postsynaptic cell membrane, postsynaptic density, synapse |
| Calculated MW: | 29kDa/33kDa |
| Observed MW: | 30kDa |
| Synonyms: | CDK5, LIS7, PSSALRE |
| Background: | This gene encodes a proline-directed serine/threonine kinase that is a member of the cyclin-dependent kinase family of proteins. Unlike other members of the family, the protein encoded by this gene does not directly control cell cycle regulation. Instead the protein, which is predominantly expressed at high levels in mammalian postmitotic central nervous system neurons, functions in diverse processes such as synaptic plasticity and neuronal migration through phosphorylation of proteins required for cytoskeletal organization, endocytosis and exocytosis, and apoptosis. In humans, an allelic variant of the gene that results in undetectable levels of the protein has been associated with lethal autosomal recessive lissencephaly-7. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | CDK5: a protein kinase of the CDK family. Unlike other members of this family, it is not activated by cyclins but by p35 (CDK5R1) and p39. An important regulator of neuronal positioning during brain development. May also play a role in synaptogenesis and neurotransmission. Substrates include TAU, MAP2, NF-H and -M, Nudel, PDE6, beta-catenin, amphyphysin, dynamin I, synapsin 1, Munc-18, and NMDA receptor 2A. Plays a role in myogenesis, haematopoietic cell differentiation, spermatogenesis, insulin secretion, and lens differentiation. Implicated in the pathology of neurofibrillary tangles and formation of senile plaques, hallmarks of Alzheimer?s disease. Induces tau phosphorylation and aggregation and neurofibrillary tangle deposition and neurodegeneration in in vitro and in vivo animal models. Brain samples from Alzeimer?s pateints show elevated CDK5 activity. |
| UniProt Protein Details: | Protein type:Protein kinase, CMGC; Kinase, protein; Cell cycle regulation; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; CMGC group; CDK family; CDK5 subfamily; CDK/CDK5 subfamily Chromosomal Location of Human Ortholog: 7q36 Cellular Component: axon; cell junction; cell soma; cytoplasm; cytosol; dendrite; growth cone; membrane; neuromuscular junction; nucleoplasm; nucleus; plasma membrane; postsynaptic density Molecular Function:acetylcholine receptor activator activity; cyclin-dependent protein kinase activity; ErbB-2 class receptor binding; ErbB-3 class receptor binding; kinase activity; protein binding; protein kinase activity; protein serine/threonine kinase activity; tau-protein kinase activity Biological Process: axon extension; cell proliferation; negative regulation of proteolysis; negative regulation of transcription, DNA-dependent; neurite development; neuron apoptosis; neuron differentiation; neuron migration; oligodendrocyte differentiation; peptidyl-serine phosphorylation; phosphorylation; positive regulation of neuron apoptosis; regulation of apoptosis; regulation of macroautophagy; regulation of synaptic plasticity; synaptic transmission; synaptic vesicle endocytosis; synaptic vesicle exocytosis; synaptogenesis Disease: Lissencephaly 7 With Cerebellar Hypoplasia |
| NCBI Summary: | This gene encodes a proline-directed serine/threonine kinase that is a member of the cyclin-dependent kinase family of proteins. Unlike other members of the family, the protein encoded by this gene does not directly control cell cycle regulation. Instead the protein, which is predominantly expressed at high levels in mammalian postmitotic central nervous system neurons, functions in diverse processes such as synaptic plasticity and neuronal migration through phosphorylation of proteins required for cytoskeletal organization, endocytosis and exocytosis, and apoptosis. In humans, an allelic variant of the gene that results in undetectable levels of the protein has been associated with lethal autosomal recessive lissencephaly-7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2015] |
| UniProt Code: | Q00535 |
| NCBI GenInfo Identifier: | 4033704 |
| NCBI Gene ID: | 1020 |
| NCBI Accession: | Q00535.3 |
| UniProt Secondary Accession: | Q00535,A1XKG3, |
| UniProt Related Accession: | Q00535 |
| Molecular Weight: | 29,544 Da |
| NCBI Full Name: | Cyclin-dependent-like kinase 5 |
| NCBI Synonym Full Names: | cyclin dependent kinase 5 |
| NCBI Official Symbol: | CDK5Â Â |
| NCBI Official Synonym Symbols: | LIS7; PSSALREÂ Â |
| NCBI Protein Information: | cyclin-dependent-like kinase 5 |
| UniProt Protein Name: | Cyclin-dependent-like kinase 5 |
| UniProt Synonym Protein Names: | Cell division protein kinase 5; Serine/threonine-protein kinase PSSALRE; Tau protein kinase II catalytic subunit; TPKII catalytic subunit |
| Protein Family: | CDK5RAP1-like protein |
| UniProt Gene Name: | CDK5Â Â |
| UniProt Entry Name: | CDK5_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CDK5 antibody (CAB5730) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Western blot analysis of extracts of various cell lines, using CDK5 antibody (CAB5730) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of U2OS cells using CDK5 antibody (CAB5730). Blue: DAPI for nuclear staining. |
 | Immunoprecipitation analysis of 900ug extracts of A-549 cells using 3ug CDK5 antibody (CAB5730). Western blot was performed from the immunoprecipitate using CDK5 antibody (CAB5730) at a dilition of 1:1000. |
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